Back to Search
Start Over
Glomerular Pyrimidine Metabolism in Experimental Diabetic Nephropathy
- Source :
- Kidney and Blood Pressure Research. 3:79-87
- Publication Year :
- 1980
- Publisher :
- S. Karger AG, 1980.
-
Abstract
- Glomerular uracil nucleotide metabolism was studied in vivo in control and diabetic rats 48 h after the injection of streptozotocin. The animals were infused for 2 h with 3H-orotate and the fraction of infused dpm incorporated into glomerular uracil nucleotides and RNA/mg of glomerular DNA was calculated. Diabetic glomeruli showed an increase in RNA/DNA compared to controls (p < 0.05) and a greater incorporation of 3H-orotate into uracil nucleotides and RNA. These changes were reversed by insulin therapy. In separate experiments the renal cortical uracil nucleotide pool was expanded by feeding chow supplemented with 0.5% orotate to rats for 6 months. Normal animals fed orotate developed significant glomerular basement membrane thickening (p < 0.01) when compared to age-matched controls, which was morphologically indistinguishable from that of diabetics. Orotate feeding also produced further basement membrane thickening in diabetic rats. These results suggest that early diabetes is characterized by an increase in glomerular uracil nucleotides, and that chronic expansion of the uracil nucleotide pool is associated with glomerular basement membrane thickening.
- Subjects :
- Male
medicine.medical_specialty
Kidney Cortex
Time Factors
Uracil Nucleotides
DNA biosynthesis
Kidney Glomerulus
Basement Membrane
Diabetes Mellitus, Experimental
Diabetic nephropathy
In vivo
Diabetes mellitus
Internal medicine
medicine
Animals
Orotic Acid
Chemistry
DNA
General Medicine
Metabolism
medicine.disease
Streptozotocin
Rats
Pyrimidines
Endocrinology
Nephrology
Pyrimidine metabolism
RNA
Cardiology and Cardiovascular Medicine
Uracil nucleotide
medicine.drug
Subjects
Details
- ISSN :
- 14230143 and 14204096
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Kidney and Blood Pressure Research
- Accession number :
- edsair.doi.dedup.....fce3bae9063f94fdc8a3e65ca758e02c