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The crucial role of the Pls1 tetraspanin during ascospore germination in Podospora anserina provides an example of the convergent evolution of morphogenetic processes in fungal plant pathogens and saprobes
- Source :
- Eukaryotic Cell, Eukaryotic Cell, American Society for Microbiology, 2008, 7 (10), pp.1809-18. ⟨10.1128/EC.00149-08⟩, Eukaryotic Cell 10 (7), 1809-1818. (2008)
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- Pls1 tetraspanins were shown for some pathogenic fungi to be essential for appressorium-mediated penetration into their host plants. We show here that Podospora anserina , a saprobic fungus lacking appressorium, contains PaPls1 , a gene orthologous to known PLS1 genes. Inactivation of PaPls1 demonstrates that this gene is specifically required for the germination of ascospores in P. anserina . These ascospores are heavily melanized cells that germinate under inducing conditions through a specific pore. On the contrary, MgPLS1 , which fully complements a Δ PaPls1 ascospore germination defect, has no role in the germination of Magnaporthe grisea nonmelanized ascospores but is required for the formation of the penetration peg at the pore of its melanized appressorium. P. anserina mutants with mutation of PaNox2 , which encodes the NADPH oxidase of the NOX2 family, display the same ascospore-specific germination defect as the Δ PaPls1 mutant. Both mutant phenotypes are suppressed by the inhibition of melanin biosynthesis, suggesting that they are involved in the same cellular process required for the germination of P. anserina melanized ascospores. The analysis of the distribution of PLS1 and NOX2 genes in fungal genomes shows that they are either both present or both absent. These results indicate that the germination of P. anserina ascospores and the formation of the M. grisea appressorium penetration peg use the same molecular machinery that includes Pls1 and Nox2. This machinery is specifically required for the emergence of polarized hyphae from reinforced structures such as appressoria and ascospores. Its recurrent recruitment during fungal evolution may account for some of the morphogenetic convergence observed in fungi.
- Subjects :
- Mutant
relation hôte-parasite
champignon pathogène
MESH: Amino Acid Sequence
biosynthèse
Podospora anserina
MESH: Plant Diseases
MESH: Melanins
Gene Expression Regulation, Fungal
tetraspanines Pls 1
MESH: Magnaporthe
MESH: Phylogeny
Phylogeny
MESH: Evolution, Molecular
Sequence Deletion
0303 health sciences
Podospora
Vegetal Biology
biology
General Medicine
Articles
Spores, Fungal
MESH: Sequence Deletion
Magnaporthe grisea
Spore fongique
Cell biology
Magnaporthe
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Fungal Proteins
MESH: Gene Expression Regulation, Fungal
MESH: Fungi
Hypha
Molecular Sequence Data
MESH: Sequence Alignment
Germination
Fungus
Microbiology
Evolution, Molecular
Fungal Proteins
03 medical and health sciences
podospora anserina
MESH: Spores, Fungal
MESH: Podospora
Botany
Morphogénèse
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
ascospore
Amino Acid Sequence
Molecular Biology
Transmission des maladies
030304 developmental biology
H20 - Maladies des plantes
Plant Diseases
Melanins
Appressorium
MESH: Molecular Sequence Data
030306 microbiology
fungi
Fungi
biology.organism_classification
Gène
Ascospore
Protéine végétale
Sequence Alignment
Biologie végétale
Subjects
Details
- Language :
- English
- ISSN :
- 15359778 and 15359786
- Database :
- OpenAIRE
- Journal :
- Eukaryotic Cell, Eukaryotic Cell, American Society for Microbiology, 2008, 7 (10), pp.1809-18. ⟨10.1128/EC.00149-08⟩, Eukaryotic Cell 10 (7), 1809-1818. (2008)
- Accession number :
- edsair.doi.dedup.....fcbb5d6ad49dbc75f9bb924feb4f56fe