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Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO2 capture
- Source :
- Bioresources and Bioprocessing, Vol 8, Iss 1, Pp 1-16 (2021)
- Publication Year :
- 2021
- Publisher :
- SpringerOpen, 2021.
-
Abstract
- Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key CO2-fixing enzyme in photosynthesis, is notorious for its low carboxylation. We report a highly active and assembly-competent Form II Rubisco from the endosymbiont of a deep-sea tubeworm Riftia pachyptila (RPE Rubisco), which shows a 50.5% higher carboxylation efficiency than that of a high functioning Rubisco from Synechococcus sp. PCC7002 (7002 Rubisco). It is a simpler hexamer with three pairs of large subunit homodimers around a central threefold symmetry axis. Compared with 7002 Rubisco, it showed a 3.6-fold higher carbon capture efficiency in vivo using a designed CO2 capture model. The simple structure, high carboxylation efficiency, easy heterologous soluble expression/assembly make RPE Rubisco a ready-to-deploy enzyme for CO2 capture that does not require complex co-expression of chaperones. The chemosynthetic CO2 fixation machinery of chemolithoautotrophs, CO2-fixing endosymbionts, may be more efficient than previously realized with great potential for next-generation microbial CO2 sequestration platforms.
- Subjects :
- inorganic chemicals
Oxygenase
Technology
Rubisco
Biomedical Engineering
TP1-1185
Random hexamer
Photosynthesis
chemistry.chemical_compound
Form II
Hexamer
Riftia pachyptila endosymbiont
Ribulose 1,5-bisphosphate
biology
Renewable Energy, Sustainability and the Environment
Chemistry
Chemical technology
Carbon fixation
RuBisCO
fungi
food and beverages
Pyruvate carboxylase
Biochemistry
Carboxylation
biology.protein
CO2 capture in vivo
TP248.13-248.65
Food Science
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 21974365
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Bioresources and Bioprocessing
- Accession number :
- edsair.doi.dedup.....fcb779e4a20e467fc31b17cdb8cc6fa7