Back to Search
Start Over
The Cuban Propolis Component Nemorosone Inhibits Proliferation and Metastatic Properties of Human Colorectal Cancer Cells
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 5, International Journal of Molecular Sciences, Vol 21, Iss 5, p 1827 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- The majority of deaths related to colorectal cancer (CRC) are associated with the metastatic process. Alternative therapeutic strategies, such as traditional folk remedies, deserve attention for their potential ability to attenuate the invasiveness of CRC cells. The aim of this study is to investigate the biological activity of brown Cuban propolis (CP) and its main component nemorosone (NEM) and to describe the molecular mechanism(s) by which they inhibit proliferation and metastatic potential of 2 CRC cell lines, i.e., HT-29 and LoVo. Our results show that CP and NEM significantly decreased cell viability and inhibited clonogenic capacity of CRC cells in a dose and time-dependent manner, by arresting the cell cycle in the G0/G1 phase and inducing apoptosis. Furthermore, CP and NEM downregulated BCL2 gene expression and upregulated the expression of the proapoptotic genes TP53 and BAX, with a consequent activation of caspase 3/7. They also attenuated cell migration and invasion by inhibiting MMP9 activity, increasing E-cadherin and decreasing &beta<br />catenin and vimentin expression, proteins involved in the epithelial&ndash<br />mesenchymal transition (EMT). In conclusion NEM, besides displaying antiproliferative activity on CRC cells, is able to decrease their metastatic potential by modulating EMT-related molecules. These finding provide new insight about the mechanism(s) of the antitumoral properties of CP, due to NEM content.
- Subjects :
- 0301 basic medicine
Caspase 3
colorectal cancer
Apoptosis
epithelial–mesenchymal transition
Catalysis
Article
Propolis
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Benzophenones
0302 clinical medicine
Cell Movement
Biomarkers, Tumor
Tumor Cells, Cultured
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Viability assay
Physical and Theoretical Chemistry
Clonogenic assay
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Cell Proliferation
Chemistry
Organic Chemistry
Cell Cycle
Cell migration
General Medicine
nemorosone
Cell cycle
Computer Science Applications
Gene Expression Regulation, Neoplastic
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Cell culture
030220 oncology & carcinogenesis
apoptosis cell death
Cancer research
Colorectal Neoplasms
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....fcb283fd23b43e0debb8d6533e2dcbb9