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Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays
- Source :
- Frontiers in Neuroscience, 9(412), 00412-00412. Frontiers Research Foundation, Frontiers in Neuroscience, Vol 9 (2015), Frontiers in Neuroscience
- Publication Year :
- 2015
- Publisher :
- Frontiers Media S.A., 2015.
-
Abstract
- Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber.
- Subjects :
- Materials science
Cellular level
lcsh:RC321-571
IR-98611
electrical stimulation
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
spontaneous activity
stimulus response
Original Research
business.industry
cortical neurons
General Neuroscience
METIS-315074
Cortical neurons
electrophysiology
multi electrode array
Axon growth
Stimulus response
EWI-26529
Electrophysiology
stimulus response compatibility
Electrode
Optoelectronics
BSS-Neurotechnology and cellular engineering
business
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 1662453X and 16624548
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Neuroscience
- Accession number :
- edsair.doi.dedup.....fcb15099f85ea2ee956032e46bf9359f
- Full Text :
- https://doi.org/10.3389/fnins.2015.00412