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The Impact on Infectivity and Neutralization Efficiency of SARS-CoV-2 Lineage B.1.351 Pseudovirus
- Source :
- Viruses, Viruses, Vol 13, Iss 633, p 633 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- A new variant of SARS-CoV-2 B.1.351 lineage (first found in South Africa) has been raising global concern due to its harboring of multiple mutations in the spike that potentially increase transmissibility and yield resistance to neutralizing antibodies. We here tested infectivity and neutralization efficiency of SARS-CoV-2 spike pseudoviruses bearing particular mutations of the receptor-binding domain (RBD) derived either from the Wuhan strains (referred to as D614G or with other sites) or the B.1.351 lineage (referred to as N501Y, K417N, and E484K). The three different pseudoviruses B.1.351 lineage related significantly increased infectivity compared with other mutants that indicated Wuhan strains. Interestingly, K417N and E484K mutations dramatically enhanced cell–cell fusion than N501Y even though their infectivity were similar, suggesting that K417N and E484K mutations harboring SARS-CoV-2 variant might be more transmissible than N501Y mutation containing SARS-CoV-2 variant. We also investigated the efficacy of two different monoclonal antibodies, Casirivimab and Imdevimab that neutralized SARS-CoV-2, against several kinds of pseudoviruses which indicated Wuhan or B.1.351 lineage. Remarkably, Imdevimab effectively neutralized B.1.351 lineage pseudoviruses containing N501Y, K417N, and E484K mutations, while Casirivimab partially affected them. Overall, our results underscore the importance of B.1.351 lineage SARS-CoV-2 in the viral spread and its implication for antibody efficacy.
- Subjects :
- 0301 basic medicine
Lineage (genetic)
medicine.drug_class
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Mutant
Casirivimab
lcsh:QR1-502
B.1.351
Molecular Dynamics Simulation
Antibodies, Monoclonal, Humanized
Antibodies, Viral
medicine.disease_cause
Monoclonal antibody
Neutralization
lcsh:Microbiology
South Africa
03 medical and health sciences
Virology
Chlorocebus aethiops
0502 economics and business
medicine
viral infectivity
Animals
Humans
Vero Cells
Infectivity
Mutation
biology
SARS-CoV-2
Communication
05 social sciences
Antibodies, Monoclonal
COVID-19
neutralization
Antibodies, Neutralizing
K417N and E484K mutation of spike
HEK293 Cells
030104 developmental biology
Infectious Diseases
Spike Glycoprotein, Coronavirus
biology.protein
050211 marketing
Angiotensin-Converting Enzyme 2
Antibody
Imdevimab
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 13
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....fcae4370d672389cb1ea4e58ea7f87e5