Back to Search
Start Over
Human Metaplastic Breast Carcinoma and Decorin
- Source :
- Cancer Microenvironment
- Publication Year :
- 2017
- Publisher :
- Springer Netherlands, 2017.
-
Abstract
- Metaplastic breast carcinoma (MBC) is a rare subtype of invasive breast cancer and has poor prognosis. In general, cancers are heterogeneous cellular masses comprised of different cell types and their extracellular matrix (ECM). However, little is known about the composition of the ECM and its constituents in MBC. Decorin is a ubiquitous ECM macromolecule known of its oncosuppressive activity. As such, it provides an intriguing molecule in the development of novel therapeutics for different malignancies such as MBC. In this study, decorin immunoreactivity and the effect of adenoviral decorin cDNA (Ad-DCN) transduction were examined in MBC. Multiple immunohistochemical stainings were used to characterize a massive breast tumour derived from an old woman. Furthermore, three-dimensional (3D) explant cultures derived from the tumour were transduced with Ad-DCN to study the effect of the transduction on the explants. The MBC tumour was shown to be completely negative for decorin immunoreactivity demonstrating that the malignant cells were not able to synthesize decorin. Ad-DCN transduction resulted in a markedly altered cytological phenotype of MBC explants by decreasing the amount of atypical cells and by inhibiting cell proliferation. The results of this study support approaches to develop new, decorin-based adjuvant therapies for MBC. Electronic supplementary material The online version of this article (doi:10.1007/s12307-017-0195-8) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell type
Pathology
medicine.medical_specialty
Decorin
Biology
Extracellular matrix
03 medical and health sciences
Transduction (genetics)
0302 clinical medicine
Breast cancer
medicine
Metaplastic breast carcinoma
skin and connective tissue diseases
Three-dimensional (3D) explant culture
Cell growth
Adenoviral transduction
Metaplastic Breast Carcinoma
medicine.disease
carbohydrates (lipids)
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
Immunohistochemistry
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 18752284 and 18752292
- Volume :
- 10
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- Cancer Microenvironment
- Accession number :
- edsair.doi.dedup.....fc668003c6f24b1da1eb81afa540d4fe