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Induction by TNF-α of IL-6 and IL-8 in cystic fibrosis bronchial IB3-1 epithelial cells encapsulated in alginate microbeads
- Source :
- Journal of Biomedicine and Biotechnology, Università degli studi di Ferrara-IRIS, Journal of Biomedicine and Biotechnology, Vol 2010 (2010)
- Publication Year :
- 2010
-
Abstract
- We have developed a microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells. The applied method is based on generation of monodisperse droplets by a vibrational nozzle. Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath. We have found that the microencapsulation procedure does not alter the viability of the encapsulated IB3-1 cells. The encapsulated IB3-1 cells were characterized in term of secretomic profile, analyzing the culture medium by Bio-Plex strategy. The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent. In order to determine the biotechnological applications of this procedure, we determined whether encapsulated IB3-1 cells could be induced to pro-inflammatory responses, after treatment with TNF-α. In this experimental set-up, encapsulated and free IB3-1 cells were treated with TNF-α, thereafter the culture media from both cell populations were collected. As expected, TNF-αinduced a sharp increase in the secretion of interleukins, chemokines and growth factors. Of great interest was the evidence that induction of interleukin-6 and interleukin-8 occurs also by encapsulated IB3-1 cells.
- Subjects :
- Chemokine
Pathology
Cystic Fibrosis
Proteome
Health, Toxicology and Mutagenesis
Cell
lcsh:Medicine
Gene Expression
Cystic fibrosis
chemistry.chemical_compound
Glucuronic Acid
Cells, Cultured
Microscopy
biology
Hexuronic Acids
Interleukin
General Medicine
Microspheres
Cell biology
medicine.anatomical_structure
Research Design
Molecular Medicine
Tumor necrosis factor alpha
Biotechnology
Research Article
medicine.medical_specialty
Article Subject
Alginates
Cell Survival
lcsh:Biotechnology
Drug Compounding
Bronchi
behavioral disciplines and activities
lcsh:TP248.13-248.65
Genetics
medicine
Humans
Secretion
Interleukin 8
Particle Size
Molecular Biology
Interleukin-6
Tumor Necrosis Factor-alpha
lcsh:R
Interleukin-8
Epithelial Cells
medicine.disease
Glucuronic acid
chemistry
biology.protein
Subjects
Details
- ISSN :
- 11107251
- Volume :
- 2010
- Database :
- OpenAIRE
- Journal :
- Journal of biomedicinebiotechnology
- Accession number :
- edsair.doi.dedup.....fc60c2bb9edf335e37fb129bc2efb90d