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Efficacy of the amphibian peptide distinctin in a neutropenic mouse model of staphylococcal sepsis
- Source :
- Critical Care Medicine. 36:2629-2633
- Publication Year :
- 2008
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2008.
-
Abstract
- To investigate the efficacy of distinctin in a neutropenic mouse model of staphylococcal sepsis.Prospective, randomized, and controlled animal study.Research laboratory in a University Hospital.BALB/c male mice.Mice were rendered neutropenic by injecting cyclophosphamide (200 mg/kg of body weight/day) on days -4 and -2 preinfection. Infection was induced at time 0 by intraperitoneal injection of 1 x 10(9) colony forming units of the staphylococcal strain. For each model, all animals were randomized to receive intravenous isotonic sodium chloride solution, 1 mg/kg distinctin, and 10 mg/kg imipenem, 10 mg/kg vancomycin, 10 mg/kg teicoplanin or 10 mg/kg linezolid alone, or combined with 1 mg/kg distinctin.Lethality, bacterial growth in blood and peritoneum, spleen, liver, and mesenteric lymph nodes.All combined regimen showed lower lethality rates than singly treated-groups. Distinctin plus vancomycin or teicoplanin exerted the lowest lethality rate. All regimens were significantly superior to controls at reducing blood, spleen, peritoneum, liver and mesenteric lymph node complex bacterial burdens, whereas all combined treated groups were higher effective than singly treated groups.Our data indicate that distinctin alone or combined with other antibiotics may be useful in treating severe staphylococcal infections.
- Subjects :
- Male
Staphylococcus aureus
Neutropenia
Peptide
Microbial Sensitivity Tests
Critical Care and Intensive Care Medicine
medicine.disease_cause
Staphylococcal infections
Hemolysis
Amphibian Proteins
Sepsis
Mice
Random Allocation
Pharmacotherapy
Intensive care
Animals
Medicine
chemistry.chemical_classification
Mice, Inbred BALB C
Leukopenia
Dose-Response Relationship, Drug
business.industry
Staphylococcal Infections
medicine.disease
Anti-Bacterial Agents
Disease Models, Animal
chemistry
Immunology
Drug Therapy, Combination
Anura
medicine.symptom
business
Antimicrobial Cationic Peptides
Subjects
Details
- ISSN :
- 00903493
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Critical Care Medicine
- Accession number :
- edsair.doi.dedup.....fc60aa51e7ae262b7574c0023a57b97e