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Screening for novel laccase-producing microbes
- Source :
- Kiiskinen, L-L, Rättö, M & Kruus, K 2004, ' Screening for novel laccase-producing microbes ', Journal of Applied Microbiology, vol. 97, no. 3, pp. 640-646 . https://doi.org/10.1111/j.1365-2672.2004.02348.x
- Publication Year :
- 2004
-
Abstract
- Aims: To discover novel laccases potential for industrial applications. Methods and Results: Fungi were cultivated on solid media containing indicator compounds that enabled the detection of laccases as specific colour reactions. The indicators used were Remazol Brilliant Blue R (RBBR), Poly R-478, guaiacol and tannic acid. The screening work resulted in isolation of 26 positive fungal strains. Liquid cultivations of positive strains confirmed that four efficient laccase producers were found in the screening. Biochemical characteristics of the four novel laccases were typical for fungal laccases in terms of molecular weight, pH optima and pI. The laccases showed good thermal stability at 60°C. Conclusions: Plate-test screening based on polymeric dye compounds, guaiacol and tannic acid is an efficient way to discover novel laccase producers. The results indicated that screening for laccase activity can be performed with guaiacol and RBBR or Poly R-478. Significance and Impact of the Study: Laccases have many potential industrial applications including textile dye decolourization, delignification of pulp and effluent detoxification. It is essential to find novel, efficient enzymes to further develop these applications. This study showed that relatively simple plate test screening method can be used for discovery of novel laccases.
- Subjects :
- Polymers
Anthraquinones
engineering.material
Applied Microbiology and Biotechnology
Remazol Brilliant Blue R
chemistry.chemical_compound
Industrial Microbiology
Tannic acid
Screening method
Coloring Agents
Laccase
guaiacol
Pulp (paper)
screening
Guaiacol
Fungi
Temperature
General Medicine
Industrial microbiology
Hydrogen-Ion Concentration
Combinatorial chemistry
Culture Media
tannic acid
Poly R-478
Molecular Weight
RBBR
chemistry
Biochemistry
laccase, Poly R-478
engineering
Indicators and Reagents
Tannins
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Kiiskinen, L-L, Rättö, M & Kruus, K 2004, ' Screening for novel laccase-producing microbes ', Journal of Applied Microbiology, vol. 97, no. 3, pp. 640-646 . https://doi.org/10.1111/j.1365-2672.2004.02348.x
- Accession number :
- edsair.doi.dedup.....fc358f95883ebcb68401aae3ce0a14fe