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Discordance Between Immunohistochemistry and In Situ Hybridization to Detect HER2 Overexpression/Gene Amplification in Breast Cancer in the Modern Age: A Single Institution Experience and Pooled Literature Review Study
- Source :
- Clinical breast cancer. 22(1)
- Publication Year :
- 2021
-
Abstract
- Background Human epidermal growth factor 2 (HER2) amplification and/or overexpression occurs in 12% to 25% of breast cancers. Accurate detection of HER2 is critical in predicting response to HER2-targeted therapy. Both immunohistochemistry (IHC) and in situ hybridization (ISH) are FDA-approved methods for detecting HER2 status because its protein overexpression is largely attributable to gene amplification. However, variable discordant results between IHC and ISH have been reported. Methods We determined the frequency of HER2 IHC/ISH discordance in these patients and also performed a pooled literature review analysis. Results Of the 1125 consecutive primary or metastatic breast cancers with HER2 IHC and ISH performed simultaneously between 2015 and 2020, 84.6% had an unequivocal HER2 status. Discordance was found in 30 cases from 26 patients, including 13 IHC−/ISH+ and 17 IHC+/ISH−, representing 1.6% and 11.9% of IHC− and IHC+ cases, respectively. Review of the literature between 2001 and 2020 identified 46 relevant studies, with a total of 43,468 cases with IHC and ISH performed. The IHC−/ISH+ and IHC+/ISH− discordances were seen in all antibody clones and ISH methods used. The IHC+/ISH− discordance was significantly higher than IHC−/ISH+ (13.8% vs. 3%, P
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Receptor, ErbB-2
Breast Neoplasms
In situ hybridization
03 medical and health sciences
0302 clinical medicine
Breast cancer
hemic and lymphatic diseases
Gene duplication
medicine
Biomarkers, Tumor
Humans
Single institution
Diagnostic Errors
skin and connective tissue diseases
neoplasms
In Situ Hybridization
In Situ Hybridization, Fluorescence
Observer Variation
Review study
biology
business.industry
Gene Amplification
virus diseases
medicine.disease
Review analysis
Immunohistochemistry
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
biology.protein
Female
Antibody
business
Subjects
Details
- ISSN :
- 19380666
- Volume :
- 22
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Clinical breast cancer
- Accession number :
- edsair.doi.dedup.....fc35244c85d6146e60a2c724f9d980b3