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Constant mean viral copy number per infected cell in tissues regardless of high, low, or undetectable plasma HIV RNA
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1999
-
Abstract
- Quantitative analysis of the relationship between virus expression and disease outcome has been critical for understanding HIV-1 pathogenesis. Yet the amount of viral RNA contained within an HIV-expressing cell and the relationship between the number of virus-producing cells and plasma virus load has not been established or reflected in models of viral dynamics. We report here a novel strategy for the coordinated analysis of virus expression in lymph node specimens. The results obtained for patients with a broad range of plasma viral loads before and after antiretroviral therapy reveal a constant mean viral (v)RNA copy number (3.6 log10 copies) per infected cell, regardless of plasma virus load or treatment status. In addition, there was a significant but nonlinear direct correlation between the frequency of vRNA+ lymph node cells and plasma vRNA. As predicted from this relationship, residual cells expressing this same mean copy number are detectable (frequency
- Subjects :
- lymph node biopsy
viruses
Biopsy
Immunology
Viremia
Cell Count
HIV Infections
In situ hybridization
Biology
HIV-1 infection
Virus Replication
Antiviral Agents
Virus
Monocytes
medicine
Immunology and Allergy
Humans
Lymph node
quantitative RT-PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA
Articles
Viral Load
medicine.disease
Virology
Molecular biology
Lymphatic system
medicine.anatomical_structure
HIV pathogenesis
Viral replication
HIV-1
RNA, Viral
Lymph Nodes
in situ hybridization
Viral load
Subjects
Details
- ISSN :
- 00221007
- Volume :
- 189
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The Journal of experimental medicine
- Accession number :
- edsair.doi.dedup.....fc1e49a77bf5ac5cb6b565a11e778f44