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Lacking hypoxia-mediated downregulation of E-cadherin in cancers of the uterine cervix
- Source :
- British Journal of Cancer
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Background: Experimental studies have established a causal connection between tumour hypoxia, hypoxia-associated proteome changes and downregulation of E-cadherin, the final common pathway of epithelial-to-mesenchymal transition (EMT). Our study aimed at elucidating the interrelationship of these processes in cancers of the uterine cervix in vivo. Methods: Tumour oxygenation was assessed in 48 squamous cell carcinomas (SCC) of the uterine cervix using polarographic needle electrodes. The expression pattern of E-cadherin was investigated by immunohistochemistry and western blotting, and was compared with that of the hypoxia-inducible proteins glucose transporter (GLUT)-1 and carbonic anhydrase (CA) IX in biopsy specimens of the oxygenation measurement tracks. Results: The majority of cervical cancers (52%) were E-cadherin positive, with a complete absence of the antigen in only 10% of the tumours. No correlation was found between the level of E-cadherin expression and the oxygenation status (mean pO2, median pO2 and hypoxic fractions). In patients showing partial expression of E-cadherin (38%), staining was not preferentially diminished in GLUT-1- or CA IX-positive areas, and loss of E-cadherin occurred independently of tumour cell scattering. Conclusion: Our data provide no evidence in favour of a hypoxia-induced EMT as a mechanistic basis of cervical cancer invasiveness.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Cell
Down-Regulation
Uterine Cervical Neoplasms
Cervix Uteri
Biology
Downregulation and upregulation
Antigens, Neoplasm
Biopsy
uterine cervix cancers
Biomarkers, Tumor
medicine
Carcinoma
Humans
Epithelial–mesenchymal transition
Carbonic Anhydrase IX
Molecular Diagnostics
Carbonic Anhydrases
Glucose Transporter Type 1
medicine.diagnostic_test
Cadherin
GLUT-1
Glucose transporter
E-cadherin
Middle Aged
Cadherins
medicine.disease
Cell Hypoxia
Ki-67 Antigen
medicine.anatomical_structure
Oncology
CA IX
Carcinoma, Squamous Cell
tumour hypoxia
Immunohistochemistry
Female
tumour invasiveness
Subjects
Details
- ISSN :
- 15321827 and 00070920
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....fc17add09e368c5bc04435aea6aa6dbf