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Immunophenotyping of chicken peripheral blood lymphocyte subpopulations: individual variability and repeatability
- Source :
- Veterinary immunology and immunopathology. 125(3-4)
- Publication Year :
- 2008
-
Abstract
- T-cell lymphocyte populations can be delineated into subsets based on expression of cell surface proteins that can be measured in peripheral blood by monoclonal antibodies and flow cytometry percentages of the lymphocyte subpopulations. In order to accurately assess immunocompetence in birds, natural variability in both avian immune function and the methodology must be understood. Our objectives were to (1) further develop flow cytometry for estimating subpopulations of lymphocytes in peripheral blood from poultry, (2) estimate repeatability and variability in the methodology with respect to poultry in a free-range and environmentally diverse situation, and (3) estimate the best antibody and cell marker combination for estimating lymphocyte subpopulations. This work demonstrated the repeatability of using flow cytometry for measurements of peripheral blood in chickens using anti-chicken antibodies for lymphocyte subpopulations. Immunofluorescence staining of cells isolated from peripheral blood revealed that the CD3(+) antibodies reacted with an average of approximately 12-24% of the lymphoid cells in the blood, depending on the fluorescence type. The CD4(+) and CD8(+) molecules were expressed in a range of 4-31% and 1-10% of the lymphoid cells in the blood, respectively. Both fluorescence label and antibody company contribute to the variability of results and should be considered in future flow cytometry studies in poultry.
- Subjects :
- medicine.medical_specialty
medicine.drug_class
Lymphocyte
T-Lymphocytes
Immunology
Monoclonal antibody
Statistics, Nonparametric
Flow cytometry
Immunophenotyping
Random Allocation
Antigen
Antigens, CD
T-Lymphocyte Subsets
Internal medicine
medicine
Animals
Hematology
General Veterinary
medicine.diagnostic_test
biology
Reproducibility of Results
Flow Cytometry
Molecular biology
medicine.anatomical_structure
Peripheral blood lymphocyte
biology.protein
Female
Antibody
Chickens
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 125
- Issue :
- 3-4
- Database :
- OpenAIRE
- Journal :
- Veterinary immunology and immunopathology
- Accession number :
- edsair.doi.dedup.....fc0a75130e8b1d5032cafe8d740c3921