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Electrophysiological identification of principal and intercalated cells in the rabbit outer medullary collecting duct
- Source :
- Pflugers Archiv : European journal of physiology. 409(1-2)
- Publication Year :
- 1987
-
Abstract
- Intracellular microelectrode techniques were used together with inhibitors of Na+ transport (amiloride) and H+ transport (acetazolamide and SITS) to identify principal cells and intercalated cells in the outer stripe of the rabbit outer medullary collecting duct. The principal cell (n = 9) had a basolateral membrane voltage (Vbl) of -64.7 +/- 3.2 mV, a fractional resistance of the apical membrane (fRa = Ra/Ra + Rbl) of 0.82 +/- 0.02, and a K+-selective basolateral membrane. Luminal amiloride hyperpolarized Vbl by 10.3 +/- 2.1 mV and increased fRa to near unity (n = 7). Bath acetazolamide and SITS were without effect on these parameters. The intercalated cell (n = 5) had a Vbl of -25.0 +/- 3.2 mV, a fRa of 0.99 +/- 0.01, and a Cl(-)-selective basolateral membrane. Bath acetazolamide or SITS hyperpolarized Vbl by 26.4 +/- 8.2 mV. Luminal amiloride did not alter Vbl of this cell. The differential effects of the inhibitors also indicate that the principal and intercalated cells are probably not directly coupled electrically.
- Subjects :
- medicine.medical_specialty
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Physiology
Clinical Biochemistry
In Vitro Techniques
Membrane Potentials
Amiloride
Physiology (medical)
Internal medicine
medicine
Animals
Intercalated Cell
Kidney Tubules, Collecting
Epithelial polarity
Lagomorpha
biology
Chemistry
Sodium
Biological Transport
Apical membrane
biology.organism_classification
Acetazolamide
Electrophysiology
Endocrinology
Kidney Tubules
Biophysics
Female
Rabbits
Protons
Microelectrodes
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 00316768
- Volume :
- 409
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Pflugers Archiv : European journal of physiology
- Accession number :
- edsair.doi.dedup.....fc0091775921ea9f393191696fd628f8