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Development of human Th1 and Th2 cytokine responses: The cytokine production profile of T cells is dictated by the primaryin vitro stimulus
- Source :
- European Journal of Immunology. 23:1-5
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- Regulation of interleukin (IL)-4 production, but not IL-2 production, was found to be quite different in either freshly isolated T cells or T cell clones. Both fresh T cells and T helper 2-like clones produced IL-4 when stimulated with anti-CD2 in combination with anti-CD28. However, whereas T cell clones showed enhanced IL-4 production when phorbol 12-myristate 13-acetate (PMA) was used in addition to anti-CD2 and anti-CD28, IL-4 production by fresh T cells was inhibited by the presence of PMA. Prestimulation of fresh T cells led to the following observations: (a) activation in the absence of PMA led to a reversal of the PMA effect and (b) within 2 days these cells resembled T cell clones in that IL-4 production was no longer inhibited by PMA. When prestimulation was carried out in the presence of PMA, the inhibition of IL-4 production seemed irreversible. Removal of PMA after 3 days did not lead to renewed capability of IL-4 production, whereas IL-2 production was unimpaired. Our data show that the capacity of cultured T cells to produce IL-4 is determined and fixed during the first 2-3 days of stimulation.
- Subjects :
- Antigens, Differentiation, T-Lymphocyte
medicine.medical_specialty
medicine.medical_treatment
T cell
Immunology
CD2 Antigens
Stimulation
Biology
Lymphocyte Activation
chemistry.chemical_compound
CD28 Antigens
Antigens, CD
Internal medicine
medicine
Humans
Immunology and Allergy
Receptors, Immunologic
Cells, Cultured
Interleukin 4
CD28
Interleukin
T-Lymphocytes, Helper-Inducer
T lymphocyte
Molecular biology
Cytokine
medicine.anatomical_structure
Endocrinology
chemistry
Phorbol
Interleukin-2
Tetradecanoylphorbol Acetate
Interleukin-4
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....fbf7808d6337e9bf3586edd6d08abf6b