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Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the AscidianCiona intestinalis
- Source :
- Molecular Biology of the Cell. 14:774-785
- Publication Year :
- 2003
- Publisher :
- American Society for Cell Biology (ASCB), 2003.
-
Abstract
- Axonemes are highly organized microtubule-based structures conserved in many eukaryotes. In an attempt to study axonemes by a proteomics approach, we selectively cloned cDNAs of axonemal proteins by immunoscreening the testis cDNA library from the ascidianCiona intestinalis by using an antiserum against whole axonemes. We report here a 37-kDa protein of which cDNA occurred most frequently among total positive clones. This protein, named LRR37, belongs to the class of SDS22+ leucine-rich repeat (LRR) family. LRR37 is different from the LRR outer arm dynein light chain reported inChlamydomonas and sea urchin flagella, and thus represents a novel axonemal LRR protein. Immunoelectron microscopy by using a polyclonal antibody against LRR37 showed that it is localized on the tip of the radial spoke, most likely on the spoke head. The LRR37 protein in fact seems to form a complex together with radial spoke protein 3 in a KI extract of the axonemes. These results suggest that LRR37 is a component of the radial spoke head and is involved in the interaction with other radial spoke components or proteins in the central pair projection.
- Subjects :
- Male
DNA, Complementary
Recombinant Fusion Proteins
Immunoelectron microscopy
Blotting, Western
Molecular Sequence Data
Dynein
Leucine-rich repeat
Biology
Article
Radial spoke
Leucine
Complementary DNA
Testis
Animals
Electrophoresis, Gel, Two-Dimensional
Ciona intestinalis
Amino Acid Sequence
Microscopy, Immunoelectron
Molecular Biology
Gene Library
Base Sequence
Sequence Homology, Amino Acid
cDNA library
Radial spoke head
Cell Biology
biology.organism_classification
Immunohistochemistry
Molecular biology
Protein Structure, Tertiary
Cell biology
Microscopy, Fluorescence
Flagella
Chromatography, Gel
Carrier Proteins
Protein Binding
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....fbf38d28515d997acd9dcdb669b86615