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Expression and prognostic significance of Livin in gastric cancer
- Source :
- Oncology Reports. 30:2520-2528
- Publication Year :
- 2013
- Publisher :
- Spandidos Publications, 2013.
-
Abstract
- Livin is one of the most important members of the inhibitor of apoptosis protein family. It is overexpressed in several types of tumors and may have prognostic significance. The present study investigated the biological role of Livin in the oncogenic behavior of gastric cancer cells, the expression of Livin in gastric cancer tissue and the relationship of its expression with various clinicopathological parameters and patient survival. Small interfering RNA blocked Livin gene expression in AGS and SNU638 human gastric cancer cell lines. The expression of Livin was investigated in gastric cancer tissues by RT-PCR, western blotting and immunohistochemistry. The associations with various clinicopathological parameters and survival were analyzed. Livin knockdown inhibited tumor cell migration, invasion and proliferation in AGS and SNU638 cells. Livin knockdown induced apoptosis by activating caspase-3, caspase-7 and PARP. Livin knockdown induced cell cycle arrest by a decrease in cyclin D1, cyclin-dependent kinase 4 and 6 and an increase in expression of p21 and p27. The ERK1/2 and JNK signaling pathways were inhibited by Livin knockdown. Livin expression was upregulated in gastric cancer tissues at the mRNA and protein levels. However, no significant correlation was found between Livin expression and various clinicopathological parameters including survival. In conclusion, Livin expression may be important in the alteration of invasive and oncogenic phenotypes of gastric cancer cells. The prognostic relevance of Livin remains unclear.
- Subjects :
- Adult
Male
Oncology
Cancer Research
medicine.medical_specialty
Apoptosis
Biology
medicine.disease_cause
Inhibitor of apoptosis
Inhibitor of Apoptosis Proteins
Cyclin D1
Cell Movement
Stomach Neoplasms
Internal medicine
medicine
Humans
Neoplasm Invasiveness
RNA, Messenger
Adaptor Proteins, Signal Transducing
Aged
Cell Proliferation
Aged, 80 and over
Gene knockdown
Oncogene
Cancer
General Medicine
Middle Aged
Cell cycle
Prognosis
medicine.disease
Survival Analysis
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Cancer cell
Cancer research
Female
Carcinogenesis
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....fbf2d00e915b03cf2bf340539a9f2e7c