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Mesenchymal stem cell survival in the infarcted heart is enhanced by lentivirus vector-mediated heat shock protein 27 expression
- Publication Year :
- 2013
- Publisher :
- Mary Ann Liebert Inc, 2013.
-
Abstract
- Mesenchymal stem cell (MSC) therapy offers the potential to promote recovery after myocardial infarction (MI). However, therapeutic efficacy may be limited by poor survival and retention of transplanted cells. A combination of gene and cell therapy has the capacity to prevent donor cell death and augment the reparative and regenerative effects of cell transfer. The present study investigates the effect of exogenous heat shock protein 27 (Hsp27) expression in MSCs in an in vitro model of ischemia and in an in vivo rat MI model and aims to determine if this could enhance the therapeutic benefit associated with cell delivery. Hsp27 overexpression by lentivirus vector modification resulted in increased MSC survival in vitro and in vivo. Furthermore, decreased apoptosis in the infarcted tissue and improved cardiac function was observed in the Hsp27 group, enhancing the therapeutic effect of MSCs. Together, these data demonstrate that ex vivo genetic modification-specifically Hsp27 overexpression-offers the possibility of enhancing the efficacy of MSC therapy in MI.
- Subjects :
- Male
Cell Survival
akt
Cellular differentiation
Genetic Vectors
Cell
HSP27 Heat-Shock Proteins
Myocardial Infarction
Gene Expression
Biology
Mesenchymal Stem Cell Transplantation
Cell therapy
Hsp27
Transduction, Genetic
In vivo
marrow stromal cells
Heat shock protein
death
Genetics
medicine
Animals
Humans
acute myocardial-infarction
Transgenes
gene
Molecular Biology
Research Articles
Lentivirus
Mesenchymal stem cell
ischemic-heart
apoptosis
Cell Differentiation
Mesenchymal Stem Cells
Genetic Therapy
progenitor cells
Molecular biology
Rats
Disease Models, Animal
medicine.anatomical_structure
Cancer research
biology.protein
Molecular Medicine
cardiac repair
Ex vivo
transplantation
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....fbf1332e97c2e5850566c55ae0f8b32c