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A novel paper MAP method for rapid high resolution histological analysis
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma.
- Subjects :
- Male
Proteome
Computer science
Science
Mice, Nude
High resolution
Apoptosis
Diseases
Computational biology
Article
Mice
Imaging, Three-Dimensional
Alzheimer Disease
Tumor Cells, Cultured
Animals
Humans
Spinal Cord Injuries
Cell Proliferation
Microscopy, Confocal
Multidisciplinary
Molecular medicine
Brain Neoplasms
Biological techniques
Brain
Xenograft Model Antitumor Assays
Visualization
Neurology
Medicine
Glioblastoma
Biotechnology
Neuroscience
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....fbd83b739a4b075f6cd241d935ff46f9