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Enzyme-Linked Immunosorbent Spot (ELISpot) monitoring of cytokine-producing cells for the prediction of acute rejection in renal transplant patients
- Source :
- European cytokine network. 28(3)
- Publication Year :
- 2017
-
Abstract
- The purpose of this study was to evaluate T-cell immunity markers using serial post-transplantation monitoring of cytokine-producing cells during the first post-transplant months for the prediction of acute rejection and potentially chronic rejection of kidney allograft. We followed 57 kidney allograft recipients for meanly 3 years post-transplantation. Blood samples were collected pre-transplant, 2, 4 and 12 weeks post-transplant. The frequencies of IL-10-, IL-17- and IFN-γ-producing cells were determined in all time-points using ELISPOT assay. The results of ELISpot monitoring and levels of IL-23 and TGF-β were compared between recipients with acute (n = 12) or chronic rejection episodes and patients with stable graft function (n = 45). In all post-transplant time-points, significantly high frequencies of IFN-γ- and IL-17-producing cells and low frequency of IL-10-producing cells were observed in rejection group versus patients with stable graft function (P 0.0001). The ROC curve analysis for determining the reliability of cytokine-producing cells for the prediction of acute rejection revealed that AUC was 0.046 for IL-10 (P 0.001), 0.927 for IL-17 (P 0.001) and 0.929 for INF-γ-producing cells (P 0.001). Our results indicate that analyzing the frequencies of INF-γ/IL-10/IL-17-producing cells may define a reliable panel for the prediction of acute rejection within the first post-transplant year which could also be applicable for the prediction of chronic rejection episodes.
- Subjects :
- Adult
Graft Rejection
Male
medicine.medical_specialty
Enzyme-Linked Immunospot Assay
medicine.medical_treatment
Clinical Biochemistry
Immunology
030204 cardiovascular system & hematology
030230 surgery
Gastroenterology
Interleukin-23
03 medical and health sciences
Interferon-gamma
0302 clinical medicine
Immunity
Transforming Growth Factor beta
Internal medicine
medicine
Immunology and Allergy
Humans
Kidney transplantation
chemistry.chemical_classification
Kidney
business.industry
ELISPOT
Interleukin-17
Middle Aged
medicine.disease
Molecular medicine
Kidney Transplantation
Interleukin-10
Enzyme
medicine.anatomical_structure
Cytokine
chemistry
Renal transplant
Cytokines
Female
business
Subjects
Details
- ISSN :
- 19524005
- Volume :
- 28
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- European cytokine network
- Accession number :
- edsair.doi.dedup.....fbd52c66e241e9d1726ec1926df04c92