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Phenomics reveals a novel putative chloroplast fatty acid transporter in the marine diatom Skeletonema marinoi involved in temperature acclimation
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Diatoms are the dominant phytoplankton in temperate oceans and coastal regions and yet little is known about the genetic basis underpinning their global success. Here, we address this challenge by developing the first phenomic approach for a diatom, screening a collection of randomly mutagenized but identifiably tagged transformants. Based upon their tolerance to temperature extremes, several compromised mutants were identified revealing genes either stress related or encoding hypothetical proteins of unknown function. We reveal one of these hypothetical proteins is a novel putative chloroplast fatty acid transporter whose loss affects several fatty acids including the two omega-3, long-chain polyunsaturated fatty acids - eicosapentaenoic and docosahexaenoic acid, both of which have medical importance as dietary supplements and industrial significance in aquaculture and biofuels. This mutant phenotype not only provides new insights into the fatty acid biosynthetic pathways in diatoms but also highlights the future value of phenomics for revealing specific gene functions in these ecologically important phytoplankton.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cell biology
Chloroplasts
Acclimatization
lcsh:Medicine
01 natural sciences
Article
03 medical and health sciences
Phenomics
Transformation, Genetic
Skeletonema marinoi
lcsh:Science
Gene
Ecosystem
chemistry.chemical_classification
Genetics
Diatoms
Multidisciplinary
Genome
biology
Fatty Acids
fungi
lcsh:R
Temperature
Fatty acid
Membrane Transport Proteins
biology.organism_classification
Chloroplast
Mutagenesis, Insertional
030104 developmental biology
Diatom
chemistry
Docosahexaenoic acid
Mutagenesis
Mutation
lcsh:Q
010606 plant biology & botany
Polyunsaturated fatty acid
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....fbd25d0b54527b4914136da4ddf42436