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Role of IFN-gamma and LPS on neuron/glial co-cultures infected by Neospora caninum
- Source :
- Repositório Institucional da UFBA, Universidade Federal da Bahia (UFBA), instacron:UFBA, Frontiers in Cellular Neuroscience, Vol 8 (2014), Frontiers in Cellular Neuroscience
- Publication Year :
- 2014
- Publisher :
- Frontiers in Cellular Neuroscience, 2014.
-
Abstract
- Submitted by Ramon El-Bachá (ramon@ufba.br) on 2016-09-30T20:33:12Z No. of bitstreams: 1 de Jesus et al 2014.pdf: 915416 bytes, checksum: 4d7c829f8d4091e11a0ce03d46824d54 (MD5) Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2016-10-07T11:54:37Z (GMT) No. of bitstreams: 1 de Jesus et al 2014.pdf: 915416 bytes, checksum: 4d7c829f8d4091e11a0ce03d46824d54 (MD5) Made available in DSpace on 2016-10-07T11:54:37Z (GMT). No. of bitstreams: 1 de Jesus et al 2014.pdf: 915416 bytes, checksum: 4d7c829f8d4091e11a0ce03d46824d54 (MD5) Previous issue date: 2014-10-27 CNPq/CAPES Neospora caninum causes cattle abortion and neurological symptoms in dogs. Although infection is usually asymptomatic, classical neurological symptoms of neosporosis may be associated with encephalitis. This parasite can grow in brain endothelial cells without markedly damages, but it can modulate the cellular environment to promote its survival in the brain. In previous studies, we described that IFN-g decreased the parasite proliferation and down regulated nitric oxide (NO) production in astrocyte/microglia cultures. However,it remains unclear how glial cells respond to N. caninum in the presence of neurons. Therefore, we evaluated the effect of 300 IU/mL IFN-gamma or 1.0 mg/mL of LPS on infected rat neuron/glial co-cultures. After 72 h of infection, LPS did not affect the mitochondrial dehydrogenase activity. However, IFN-gamma decreased this parameter by 15.5 and 12.0% in uninfected and infected cells, respectively. The number of tachyzoites decreased 54.1 and 44.3% in cells stimulated with IFN-gamma and LPS, respectively. Infection or LPS treatment did not change NO production. On the other hand, IFN-gamma induced increased nitrite release in 55.7%, but the infection reverted this induction. IL-10 levels increased only in infected cultures (treated or not), meanwhile PGE2 release was improved in IFN-gamma/infected or LPS/infected cells. Although IFN-gamma significantly reduced the neurite length in uninfected cultures (42.64%; p < 0.001), this inflammatory cytokine reverted the impairment of neurite outgrowth induced by the infection (81.39%). The results suggest a neuroprotective potential response of glia to N. caninum infection under IFN-gamma stimulus. This observation contributes to understand the immune mediated mechanisms of neosporosis in central nervous system (CNS).
- Subjects :
- Neurite
medicine.medical_treatment
neurite impairment
Neospora caninum
Neuroprotection
immune response
lcsh:RC321-571
Cellular and Molecular Neuroscience
Immune system
medicine
Original Research Article
Neuron/glia co-culture
parasite NO down-modulation
parasite NO downmodulation
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
biology
Microglia
neuron/glial co-culture
biology.organism_classification
medicine.anatomical_structure
Cytokine
Immunology
Neuron
Neuroscience
Astrocyte
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da UFBA, Universidade Federal da Bahia (UFBA), instacron:UFBA, Frontiers in Cellular Neuroscience, Vol 8 (2014), Frontiers in Cellular Neuroscience
- Accession number :
- edsair.doi.dedup.....fbca287befadfd6ba189d79cf3a17044
- Full Text :
- https://doi.org/10.3389/fncel.2014.00340