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Diet high in lipid hydroperoxide by vitamin E deficiency induces insulin resistance and impaired insulin secretion in normal rats
- Source :
- Diabetes research and clinical practice. 67(2)
- Publication Year :
- 2003
-
Abstract
- To clarify the effect of dietary lipid hydroperoxide (LPO) on development of glucose intolerance, we fed Sprague–Dawley rats on a diet containing elevated LPO level for 10 weeks and measured both insulin sensitivity and insulin secretion. The contents of LPO in both plasma and skeletal muscle in the LPO-fed rats were significantly higher than those in the controls. Both insulin resistance evaluated by steady-state blood glucose (SSBG) methods and impaired insulin secretion evaluated by oral glucose tolerance test (OGTT) were found in the LPO-fed rats as compared with control rats. Furthermore, the levels of insulin receptor substrate (IRS)-1 protein in the skeletal muscle were significantly lower in the LPO-fed rats. Those impairments were not reversed in LPO-fed rats with supernormal levels of plasma vitamin E following vitamin E supplementation for 5 weeks. Moreover, the immunohistochemical study revealed that NF-κB-p50 protein was found in the nucleus of pancreatic β-cells of the LPO-fed rats, whereas it was not observed in the nucleus of the islets in the control rats. These findings indicate that NF-κB is activated in response to oxidative stress in pancreatic islet cells in LPO-fed rats. In conclusion, our studies reveal that diet high in LPO by vitamin E deficiency accelerates glucose intolerance through impairments of both sensitivity and secretion of insulin.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Lipid Peroxides
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Dietary lipid
Thiobarbituric Acid Reactive Substances
Rats, Sprague-Dawley
Islets of Langerhans
Endocrinology
Insulin resistance
Diabetes mellitus
Insulin receptor substrate
Internal medicine
Glucose Intolerance
Insulin Secretion
Internal Medicine
medicine
Animals
Insulin
Vitamin E
Vitamin E Deficiency
Muscle, Skeletal
Cell Nucleus
Lipid peroxide
business.industry
NF-kappa B
General Medicine
Glucose Tolerance Test
medicine.disease
Phosphoproteins
Diet
Rats
Oxidative Stress
Insulin Receptor Substrate Proteins
Vitamin E deficiency
Insulin Resistance
business
Subjects
Details
- ISSN :
- 01688227
- Volume :
- 67
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Diabetes research and clinical practice
- Accession number :
- edsair.doi.dedup.....fba7a1adeeff208a305b2652d10601ab