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Molecular Epidemiology of Invasive Staphylococcus aureus Infections and Concordance with Colonization Isolates
- Source :
- J Pediatr
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Objectives To characterize Staphylococcus aureus isolates recovered from hospitalized children and to determine the concordance between colonizing and invasive isolates. Study design Children with culture-confirmed, community-onset, invasive S aureus infections were enrolled in this prospective case series from a large children's hospital over a 5-year period. Colonization isolates were obtained from the anterior nares, oropharynx, and inguinal folds and were compared with invasive isolates via repetitive-element, sequence-based polymerase chain reaction testing. Isolates with a ≥96% genetic match were characterized as concordant. Results A total of 86 S aureus isolates (44 invasive, 42 colonization) were collected from 44 children with invasive infections. Clinical isolates were genetically diverse, 64% of invasive isolates were methicillin-susceptible S aureus (MSSA), and 59% of cases had a colonizing S aureus isolate at the time of hospitalization. Of those who were colonized, at least 1 of their colonization isolates was indistinguishable from the infecting isolate in 88% of cases. Patients with invasive MSSA were significantly more likely to have a concordant MSSA colonization isolate present compared with patients with invasive methicillin-resistant S aureus (MRSA) (61% vs 38%, P Conclusions Invasive MSSA infection was more common than MRSA infection in this pediatric cohort, and patients with MSSA infection were significantly more likely than those with MRSA infection to have concordant colonizing isolates across multiple anatomic sites. These findings warrant larger scale validation and may have important infection control and epidemiologic implications, as unlike MRSA, transmissibility of MSSA largely is ignored in healthcare settings.
- Subjects :
- DNA, Bacterial
Male
Staphylococcus aureus
Adolescent
Concordance
New York
Oropharynx
Groin
medicine.disease_cause
Polymerase Chain Reaction
Article
law.invention
Microbiology
03 medical and health sciences
0302 clinical medicine
law
030225 pediatrics
medicine
Humans
Infection control
Colonization
Prospective Studies
030212 general & internal medicine
Child
Polymerase chain reaction
Molecular Epidemiology
Molecular epidemiology
business.industry
Infant, Newborn
Infant
Staphylococcal Infections
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Anterior nares
Community-Acquired Infections
medicine.anatomical_structure
Child, Preschool
Carrier State
Pediatrics, Perinatology and Child Health
Cohort
Female
Nasal Cavity
business
Subjects
Details
- ISSN :
- 00223476
- Volume :
- 210
- Database :
- OpenAIRE
- Journal :
- The Journal of Pediatrics
- Accession number :
- edsair.doi.dedup.....fba0662c839dc4e6a81447f32baadc3c