Back to Search
Start Over
Secondhand Tobacco Smoke Exposure Associations with DNA Methylation of the Aryl Hydrocarbon Receptor Repressor
- Source :
- Nicotine & Tobacco Research. :ntw219
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Introduction Cigarette smoking is inversely associated with DNA methylation of the aryl hydrocarbon receptor repressor (AHRR; cg05575921). However, the association between secondhand tobacco smoke (SHS) exposure and AHRR methylation is unknown. Methods DNA methylation of AHRR cg05575921 in CD14+ monocyte samples, from 495 never-smokers and 411 former smokers (having quit smoking ≥15 years) from the Multi-Ethnic Study of Atherosclerosis (MESA), was cross-sectionally compared with concomitantly ascertained self-reported SHS exposure, urine cotinine concentrations, and estimates of air pollutants at participants' homes. Linear regression was used to test for associations, and covariates included age, sex, race, education, study site, and previous smoking exposure (smoking status, time since quitting, and pack-years). Results Recent indoor SHS exposure (hours per week) was inversely associated with cg05575921 methylation (β ± SE = -0.009 ± 0.003, p = .007). The inverse effect direction was consistent (but did not reach significance) in the majority of stratified analyses (by smoking status, sex, and race). Categorical analysis revealed high levels of recent SHS exposure (≥10 hours per week) inversely associated with cg05575921 methylation (β ± SE = -0.28 ± 0.09, p = .003), which remained significant (p < .05) in the majority of stratified analyses. cg05575921 methylation did not significantly (p < .05) associate with low to moderate levels of recent SHS exposure (1-9 hours per week), urine cotinine concentrations, years spent living with people smoking, years spent indoors (not at home) with people smoking, or estimated levels of air pollutants. Conclusions High levels of recent indoor SHS exposure may be inversely associated with DNA methylation of AHRR in human monocytes. Implications DNA methylation is a biochemical alteration that can occur in response to cigarette smoking; however, little is known about the effect of SHS on human DNA methylation. In the present study, we evaluated the association between SHS exposure and DNA methylation in human monocytes, at a site (AHRR cg05575921) known to have methylation inversely associated with current and former cigarette smoking compared to never smoking. Results from this study suggest high levels of recent SHS exposure inversely associate with DNA methylation of AHRR cg05575921 in monocytes from nonsmokers, albeit with weaker effects than active cigarette smoking.
- Subjects :
- Adult
Male
0301 basic medicine
medicine.medical_treatment
Physiology
Aryl hydrocarbon receptor repressor
Urine
03 medical and health sciences
chemistry.chemical_compound
medicine
Humans
Aged
Original Investigation
Aged, 80 and over
Smoke
business.industry
Public Health, Environmental and Occupational Health
Methylation
DNA Methylation
Middle Aged
Former Smoker
030104 developmental biology
Receptors, Aryl Hydrocarbon
chemistry
DNA methylation
Smoking cessation
Female
Tobacco Smoke Pollution
business
Cotinine
Subjects
Details
- ISSN :
- 1469994X and 14622203
- Database :
- OpenAIRE
- Journal :
- Nicotine & Tobacco Research
- Accession number :
- edsair.doi.dedup.....fb979516c6b4e6f12cc6bea5b7b119e0