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Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination

Authors :
Raeven, R HM
Brummelman, J
Pennings, J.L.
Maas, L.
Helm, K.
Tilstra, W.
van der Ark, A.
Sloots, A
van der Ley, P.
van Eden, W
Jiskoot, W
van Riet, E.
van Els, C ACM
Kersten, G FA
Han, W GH
Metz, B.
LS Immunologie
LS Virologie
Dep Farmaceutische wetenschappen
dI&I RA-I&I I&I
LS Immunologie
LS Virologie
Dep Farmaceutische wetenschappen
dI&I RA-I&I I&I
Source :
Mucosal Immunology. Nature Publishing Group, Mucosal Immunology, 11(3), 979-993, Mucosal Immunology
Publication Year :
2017

Abstract

Mucosal immunity is often required for protection against respiratory pathogens but the underlying cellular and molecular mechanisms of induction remain poorly understood. Here, systems vaccinology was used to identify immune signatures after pulmonary or subcutaneous immunization of mice with pertussis outer membrane vesicles. Pulmonary immunization led to improved protection, exclusively induced mucosal immunoglobulin A (IgA) and T helper type 17 (Th17) responses, and in addition evoked elevated systemic immunoglobulin G (IgG) antibody levels, IgG-producing plasma cells, memory B cells, and Th17 cells. These adaptive responses were preceded by unique local expression of genes of the innate immune response related to Th17 (e.g., Rorc) and IgA responses (e.g., Pigr) in addition to local and systemic secretion of Th1/Th17-promoting cytokines. This comprehensive systems approach identifies the effect of the administration route on the development of mucosal immunity, its importance in protection against Bordetella pertussis, and reveals potential molecular correlates of vaccine immunity to this reemerging pathogen.Mucosal Immunology advance online publication 20 September 2017; doi:10.1038/mi.2017.81.

Details

Language :
English
ISSN :
19330219
Database :
OpenAIRE
Journal :
Mucosal Immunology. Nature Publishing Group, Mucosal Immunology, 11(3), 979-993, Mucosal Immunology
Accession number :
edsair.doi.dedup.....fb93106157a0ce384778154f113ab3bd