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A reconfigurable NAND/NOR genetic logic gate
- Source :
- BMC Systems Biology, BMC Systems Biology, Vol 6, Iss 1, p 126 (2012)
- Publication Year :
- 2012
- Publisher :
- BioMed Cental, 2012.
-
Abstract
- Background Engineering genetic Boolean logic circuits is a major research theme of synthetic biology. By altering or introducing connections between genetic components, novel regulatory networks are built in order to mimic the behaviour of electronic devices such as logic gates. While electronics is a highly standardized science, genetic logic is still in its infancy, with few agreed standards. In this paper we focus on the interpretation of logical values in terms of molecular concentrations. Results We describe the results of computational investigations of a novel circuit that is able to trigger specific differential responses depending on the input standard used. The circuit can therefore be dynamically reconfigured (without modification) to serve as both a NAND/NOR logic gate. This multi-functional behaviour is achieved by a) varying the meanings of inputs, and b) using branch predictions (as in computer science) to display a constrained output. A thorough computational study is performed, which provides valuable insights for the future laboratory validation. The simulations focus on both single-cell and population behaviours. The latter give particular insights into the spatial behaviour of our engineered cells on a surface with a non-homogeneous distribution of inputs. Conclusions We present a dynamically-reconfigurable NAND/NOR genetic logic circuit that can be switched between modes of operation via a simple shift in input signal concentration. The circuit addresses important issues in genetic logic that will have significance for more complex synthetic biology applications.
- Subjects :
- Theoretical computer science
Computer science
Logic
Population
NAND gate
03 medical and health sciences
Synthetic biology
Computers, Molecular
Structural Biology
Modelling and Simulation
Electronic engineering
Electronics
education
Molecular Biology
lcsh:QH301-705.5
030304 developmental biology
Electronic circuit
Boolean logic
Multifunctionality
0303 health sciences
education.field_of_study
Interpretation (logic)
Applied Mathematics
G400
030302 biochemistry & molecular biology
NOR logic
C400
Computer Science Applications
lcsh:Biology (General)
Modeling and Simulation
Logic gate
Synthetic Biology
Genetic Engineering
Research Article
Hardware_LOGICDESIGN
Subjects
Details
- Language :
- English
- ISSN :
- 17520509
- Database :
- OpenAIRE
- Journal :
- BMC Systems Biology, BMC Systems Biology, Vol 6, Iss 1, p 126 (2012)
- Accession number :
- edsair.doi.dedup.....fb907c94b6aa3ae8ecbe45ab9e3512e6