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Cytokeratin expression during mouse embryonic and early postnatal mammary gland development
- Source :
- Histochemistry and Cell Biology, Sun, Peng; Yuan, Yuanyang; Li, Aihua; Li, Boan; & Dai, Xing. (2010). Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochemistry and Cell Biology, 133(2), pp 213-221. doi: 10.1007/s00418-009-0662-5. Retrieved from: http://www.escholarship.org/uc/item/6mn6m7rm, Sun, P; Yuan, Y; Li, A; Li, B; & Dai, X. (2010). Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochemistry and Cell Biology, 133(2), 213-221. doi: 10.1007/s00418-009-0662-5. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/1dd2t3m2
- Publication Year :
- 2009
- Publisher :
- Springer-Verlag, 2009.
-
Abstract
- Cytokeratins are intermediate filament proteins found in most epithelial cells including the mammary epithelium. Specific cytokeratin expression has been found to mark different epithelial cell lineages and also to associate with putative mammary stem/progenitor cells. However, a comparative analysis of the expression of cytokaratins during embryonic and postnatal mammary development is currently lacking. Moreover, it is not clear whether the different classes of putative mammary stem/progenitor cells exist during embryonic development. Here, we use double/triple-label immunofluorescence and immunohistochemistry to systematically compare the expression of cytokeratin 5 (K5), cytokeratin 6 (K6),cytokeratin 8 (K8), cytokeratin 14 (K14) and cytokeratin 19 (K19) in embryonic and early postnatal mouse mammary glands. We show that K6+and K8+/K14+putative mammary progenitor cells arise during embryogenesis with distinct temporal and spatial distributions. Moreover, we describe a transient disconnection of the expression of K5 and K14, two cytokeratins that are often co-expressed, during the first postnatal weeks of mammary development. Finally, we report that cytokeratin expression in cultured primary mammary epithelial cells mimics that during the early stages of postnatal mammary development. These studies demonstrate an embryonic origin of putative mammary stem/progenitor cells. Moreover, they provide additional insights into the use of specific cytokeratins as markers of mammary epithelial differentiation, or the use of their promoters to direct gene overexpression or ablation in genetic studies of mouse mammary development. © The Author(s) 2009. This article is published with open access at Springerlink.com.
- Subjects :
- Histology
Fluorescent Antibody Technique
macromolecular substances
Biology
03 medical and health sciences
Cytokeratin
K6
Mice
0302 clinical medicine
Mammary Glands, Animal
K8
Stem and progenitor cells
Keratin
K5
medicine
Medicine & Public Health
Animals
Progenitor cell
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
Medicine/Public Health, general
0303 health sciences
Original Paper
K14
Embryogenesis
Cell Biology
Embryonic stem cell
Molecular biology
Immunohistochemistry
Epithelium
K19
Mammary gland development
Mice, Inbred C57BL
Medical Laboratory Technology
medicine.anatomical_structure
chemistry
Mammary Epithelium
030220 oncology & carcinogenesis
Keratins
Stem cell
Anatomy
Subjects
Details
- Language :
- English
- ISSN :
- 1432119X and 09486143
- Volume :
- 133
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Histochemistry and Cell Biology
- Accession number :
- edsair.doi.dedup.....fb8f265468604559c3f311c40786af72