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The analysis of cocaine and its metabolites by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS)
- Source :
- Forensic science international. 66(3)
- Publication Year :
- 1994
-
Abstract
- The method for simultaneous determination of cocaine and its four metabolites (benzoylecgonine, ecgonine methyl ester, ecgonine and norcocaine) in urine by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS) was studied. The mass spectra showed the quasi-molecular ions, [M+H]+ as the base peak. LC/APCI-MS analysis was performed by focusing the characteristic ions at m/ = 186, 290, 200, 304 and 290 for ecgonine, benzoylecgonine, ecgonine methyl ester, cocaine and norcocaine, respectively. Cocaine and its four metabolites were well separated by high performance liquid chromatography (HPLC). The recoveries of cocaine and its metabolites from the spiked urine were 40.3-94.7% by solid-phase extraction with two type cartridges (Bond Elut Certify and Bond Elut SCX).
- Subjects :
- Chemical ionization
Chromatography
Reproducibility of Results
Spectrometry, Mass, Secondary Ion
Atmospheric-pressure chemical ionization
Forensic Medicine
Mass spectrometry
High-performance liquid chromatography
Sensitivity and Specificity
Pathology and Forensic Medicine
Norcocaine
Substance Abuse Detection
chemistry.chemical_compound
chemistry
Cocaine
Liquid chromatography–mass spectrometry
Evaluation Studies as Topic
Benzoylecgonine
Humans
Ecgonine
Law
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 03790738
- Volume :
- 66
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Forensic science international
- Accession number :
- edsair.doi.dedup.....fb64a3ab23dd4a4c6c4814a0a694c52e