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Isolation of a virus closely related to gibbon ape leukaemia virus from cells infected with virus (HL-23V) released by human leukaemic cells
- Source :
- The Journal of general virology. 48(1)
- Publication Year :
- 1980
-
Abstract
- Canine thymus cells infected with virus (HL-23V) produced by human acute myelogenous leukaemia cells in culture were shown in previous reports to produce transforming and non-transforming type C virus similar or identical to the simian sarcoma virus complex SSV(SSAV) and to induce tumours in marmoset monkeys (Bergholz et al. 1977a). In these earlier studies the appearance of breakthrough foci at low dilutions of antiserum in neutralization tests with high-titred anti-SSV(SSAV) serum suggested the presence of another virus, distinct from SSV-(SSAV). We now report the isolation of this component and, by comparative neutralization analysis, demonstrate that it is most closely related to gibbon ape leukaemia virus (GALV). It is distinguished from SSV(SSAV) by kinetics of neutralization and molecular hybridization experiments. This component was readily cloned both from virus produced by HL-23V chronically-infected canine thymus cells established by Teich et al. (1975) when HL-23V was first isolated and from virus produced by HL-23V-induced marmoset tumour cells in culture. The presence of this component in the original leukaemic cell cultures is discussed.
- Subjects :
- Myeloid
viruses
Biology
Simian
Virus
Neutralization
Cell Line
Antigen
Neutralization Tests
Virology
medicine
Animals
Humans
Hylobates
Antigens, Viral
Antiserum
Sarcoma Virus, Woolly Monkey
Nucleic Acid Hybridization
medicine.disease
biology.organism_classification
Cell Transformation, Viral
Leukemia
Leukemia, Myeloid, Acute
medicine.anatomical_structure
Retroviridae
Cell culture
Satellite Viruses
Subjects
Details
- ISSN :
- 00221317
- Volume :
- 48
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of general virology
- Accession number :
- edsair.doi.dedup.....fb614dc512069b23749804e584908198