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Reversal of isoprenaline-induced cardiac remodeling by rutaecarpine via stimulation of calcitonin gene-related peptide production
- Source :
- Canadian Journal of Physiology and Pharmacology. 88:949-959
- Publication Year :
- 2010
- Publisher :
- Canadian Science Publishing, 2010.
-
Abstract
- Dysfunction of capsaicin-sensitive sensory nerves is involved in cardiac remodeling, and rutaecarpine has been shown to exert a beneficial effect on cardiac function through activating the sensory nerves. This study was conducted to explore the potential inhibitory effect of rutaecarpine on cardiac remodeling and the underlying mechanisms. A rat cardiac remodeling model was established by injection of isoprenaline (5 mg/kg per day, s.c.) for 10 days. Rutaecarpine (10 or 40 mg/kg, i.g.) was coadministrated with isoprenaline to evaluate the effect of rutaecarpine on cardiac remodeling. After echocardiographic analysis was performed, blood samples were collected to quantify calcitonin gene-related peptide (CGRP), dorsal root ganglia were isolated for examining CGRP mRNA expression, and the hearts were weighed and saved for evaluating the parameters related to apoptosis and hypertrophy. Isoprenaline significantly increased the ratio of left ventricle weight to body weight, the cross-sectional area of cardiomyocytes, cardiac apoptosis, and collagen deposition concomitantly with decreased CGRP production, which were reversed by rutaecarpine treatment. The beneficial effects of rutaecarpine were attenuated by pretreatment with capsaicin, which selectively depleted CGRP. These results suggest that rutaecarpine was able to reverse isoprenaline-induced cardiac remodeling through stimulating CGRP production.
- Subjects :
- Male
Cardiac function curve
medicine.medical_specialty
Physiology
Calcitonin Gene-Related Peptide
Heart Ventricles
Neuropeptide
Apoptosis
Stimulation
Calcitonin gene-related peptide
Indole Alkaloids
Rats, Sprague-Dawley
Ganglia, Spinal
Physiology (medical)
Internal medicine
Isoprenaline
In Situ Nick-End Labeling
medicine
Animals
Myocyte
Myocytes, Cardiac
Pharmacology
Ventricular Remodeling
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Body Weight
Isoproterenol
Organ Size
General Medicine
Rutaecarpine
Rats
Endocrinology
Echocardiography
Calcitonin
Quinazolines
medicine.drug
Subjects
Details
- ISSN :
- 12057541 and 00084212
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Physiology and Pharmacology
- Accession number :
- edsair.doi.dedup.....fb5895c4ab89d9c689c82489a46a9d1a
- Full Text :
- https://doi.org/10.1139/y10-067