Back to Search
Start Over
Association Between IHC and MSI Testing to Identify Mismatch Repair–Deficient Patients with Ovarian Cancer
- Publication Year :
- 2014
- Publisher :
- Mary Ann Liebert, Inc., 2014.
-
Abstract
- Objective: In epithelial ovarian cancer, concordance between results of microsatellite instability (MSI) and immunohistochemical (IHC) testing has not been demonstrated. This study evaluated the association of MSI-high (MSI-H) status with loss of expression (LoE) of mismatch repair (MMR) proteins on IHC and assessed for potential factors affecting the strength of the association. Methods: Tumor specimens from three population-based studies of epithelial ovarian cancer were stained for MMR proteins through manual or automated methods, and results were interpreted by one of two pathologists. Tumor and germline DNA was extracted and MSI testing performed. Multivariable logistic regression models were fitted to predict loss of IHC expression based on MSI status after adjusting for staining method and reading pathologist. Results: Of 834 cases, 564 (67.6%) were concordant; 41 were classified as MSI-H with LoE and 523 as microsatellite stable (MSS) with no LoE. Of the 270 discordant cases, 83 were MSI-H with no LoE and 187 were MSS with LoE. Both IHC staining method and reading pathologist were strongly associated with discordant results. Conclusions: Lack of concordance in the current study may be related to inconsistencies in IHC testing methods and interpretation. Results support the need for validation studies before routine screening of ovarian tumors is implemented in clinical practice for the purpose of identifying Lynch syndrome.
- Subjects :
- Oncology
medicine.medical_specialty
Pathology
endocrine system diseases
DNA Repair
DNA repair
Base Pair Mismatch
Concordance
Biology
Carcinoma, Ovarian Epithelial
Internal medicine
medicine
Carcinoma
Humans
Epithelial ovarian cancer
Neoplasms, Glandular and Epithelial
neoplasms
Genetics (clinical)
Aged
Ovarian Neoplasms
Microsatellite instability
General Medicine
Original Articles
Middle Aged
medicine.disease
Immunohistochemistry
digestive system diseases
DNA mismatch repair
Female
Microsatellite Instability
Ovarian cancer
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....fb49ee8eeb65bbd55366bbbc7e72277e