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Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells
- Source :
- International Journal of Molecular Sciences, Vol 23, Iss 1605, p 1605 (2022), International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1605
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells.
- Subjects :
- QH301-705.5
Catalysis
Inorganic Chemistry
Cell Line, Tumor
Neoplasms
Humans
Physical and Theoretical Chemistry
Biology (General)
Molecular Biology
QD1-999
Spectroscopy
Optical Imaging
Organic Chemistry
General Medicine
fluorescent imaging
Computer Science Applications
activatable peptides
proteases
MMP2
cancer cells
Chemistry
Proteolysis
Matrix Metalloproteinase 2
Peptides
HT29 Cells
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 23
- Issue :
- 1605
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....fb3e1eb7c623ca9aec17ab519a800414