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A Novel Hybrid Silk Fibroin/Polyurethane Arteriovenous Graft for Hemodialysis: Proof-of-Concept Animal Study in an Ovine Model
- Source :
- Advanced healthcare materials. 9(20)
- Publication Year :
- 2020
-
Abstract
- To solve the problem of vascular access failure, a novel semi-degradable hybrid vascular graft, manufactured by electrospinning using silk fibroin and polyurethane (Silkothane), has been previously developed and characterized in vitro. This proof-of-concept animal study aims at evaluating the performances of Silkothane grafts in a sheep model of arteriovenous shunt, in terms of patency and short-term remodeling. Nine Silkothane grafts are implanted between the common carotid artery and the external jugular vein of nine sheep, examined by palpation three times per week, by echo-color Doppler every two weeks, and euthanized at 30, 60, and 90 days (N = 3 per group). At sacrifice, grafts are harvested and submitted for histopathology and/or scanning electron microcopy (SEM). No cases of graft-related complications are recorded. Eight of nine sheep (89%) show 100% primary unassisted patency at the respective time of sacrifice (flow rate 1.76 ± 0.61 L min-1 , one case of surgery-related thrombosis excluded). Histopathology and SEM analysis evidence signs of inflammation and pseudointima inside the graft lumen, especially at the venous anastomosis; however, endoluminal stenosis never impairs the functionality of the shunt and coverage by endothelial cells is observed. In this model, Silkothane grafts grant safety and 100% patency up to 90 days.
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
Polyurethanes
Biomedical Engineering
Pharmaceutical Science
Fibroin
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
Renal Dialysis
medicine.artery
hybrid vascular grafts
medicine
Animals
Common carotid artery
hemodialysis
ovine models
silk fibroin
vascular access
Polytetrafluoroethylene
Vascular Patency
Sheep
business.industry
Graft Occlusion, Vascular
Settore ING-IND/34 - Bioingegneria Industriale
Endothelial Cells
021001 nanoscience & nanotechnology
medicine.disease
Thrombosis
0104 chemical sciences
Surgery
Blood Vessel Prosthesis
Stenosis
Histopathology
Hemodialysis
0210 nano-technology
business
Fibroins
Shunt (electrical)
External jugular vein
Subjects
Details
- ISSN :
- 21922659
- Volume :
- 9
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Advanced healthcare materials
- Accession number :
- edsair.doi.dedup.....fb3dcf179762657017798146acd02401