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miR-145-5p Suppresses Breast Cancer Progression by Inhibiting SOX2
- Source :
- Journal of Surgical Research. 236:278-287
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Background In this study, we aimed to investigate the expression and clinical significance of miR-145-5p and its tumor-suppressive effect in breast cancer patients. Methods We used luciferase reporter assay, real-time quantitative reverse transcription polymerase chain reaction and Western blot to identify sex-determining region Y-box2 (SOX2) as the target gene of miR-145-5p. Their expression levels in breast cancer tissues (n = 122) were detected by real-time quantitative polymerase chain reaction. We also applied 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry to reveal the effect of miR-145-5p on proliferation in breast cancer. Results miR-145-5p expression is downregulated in breast cancer tissues and negatively correlated with SOX2 expression. Decreased miR-145-5p expression was significantly associated with larger tumor size, distal metastasis, higher Ki67 expression level, and shorter overall survival. miR-145-5p inhibits breast cancer cell proliferation via targeting SOX2, and multivariate regression showed that both miR-145-5p and SOX2 were unfavorable prognostic factors. Conclusions miR-145-5p played a suppressive role in the proliferation of breast cancer cells by targeting SOX2, and miR-145-5p is a putative biomarker for risk assessment in patients with breast cancer.
- Subjects :
- Adult
Down-Regulation
Breast Neoplasms
Flow cytometry
Metastasis
Young Adult
03 medical and health sciences
0302 clinical medicine
Breast cancer
SOX2
Western blot
Cell Line, Tumor
Biomarkers, Tumor
medicine
Humans
Breast
Aged
Aged, 80 and over
medicine.diagnostic_test
business.industry
SOXB1 Transcription Factors
Middle Aged
Prognosis
medicine.disease
Gene Expression Regulation, Neoplastic
Reverse transcription polymerase chain reaction
MicroRNAs
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Disease Progression
Cancer research
Biomarker (medicine)
Female
030211 gastroenterology & hepatology
Surgery
business
Subjects
Details
- ISSN :
- 00224804
- Volume :
- 236
- Database :
- OpenAIRE
- Journal :
- Journal of Surgical Research
- Accession number :
- edsair.doi.dedup.....fb371431aae4c01c51c75acf0e90d37f