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Characterization and Expression of Netrin-1 and Its Receptors UNC5B and DCC in Human Placenta
- Source :
- Journal of Histochemistry & Cytochemistry. 58:73-82
- Publication Year :
- 2009
- Publisher :
- SAGE Publications, 2009.
-
Abstract
- Netrins are a family of proteins that mediate axonal guidance in the central nervous system (CNS). In addition to the CNS, netrins are involved in cell adhesion, motility, proliferation, differentiation, and survival. Because these processes occur in the placenta, we raised the question of whether netrin-1 is expressed by placental cells during development. In the present study, we analyzed the spatial and temporal distribution of netrin-1 and its two receptors, DCC (deleted in colorectal cancer) and UNC5B (uncoordinated-5 homolog) in human placenta using RT-PCR, Western blotting, and immunohistochemistry analysis. We demonstrated the presence of the proteins and transcripts of netrin-1 and its receptors in placenta and cytotrophoblasts. Furthermore, using immunohistochemistry, we localized endogenous netrin-1 protein staining to villous and extravillous cytotrophoblasts, and secreted netrin-1 outside the syncytiotrophoblasts. The DCC receptor was localized to syncytiotrophoblasts and invasive extravillous cytotrophoblasts during the first trimester and at term. On the other hand, the UNC5B receptor was localized to villous and extravillous cytotrophoblasts proximal to anchoring areas during the first trimester. At term, UNC5B was observed in decidual cells and weakly in extravillous cells. The discrete pattern of netrin-1 and netrin-1 receptor distribution suggested that netrin-1 protein functions might vary with its localization in the placenta and probably with time of gestation. (J Histochem Cytochem 58:73–82, 2010)
- Subjects :
- medicine.medical_specialty
DNA, Complementary
animal structures
Histology
Deleted in Colorectal Cancer
Cell Survival
Placenta
Cellular differentiation
Receptors, Cell Surface
Syncytiotrophoblasts
Biology
Article
Cell Movement
Pregnancy
Internal medicine
Netrin
Cell Adhesion
medicine
Humans
Decidual cells
Nerve Growth Factors
Receptor
reproductive and urinary physiology
DNA Primers
Tumor Suppressor Proteins
fungi
Gene Amplification
Abortion, Induced
Cell Differentiation
Netrin-1
DCC Receptor
Immunohistochemistry
Trophoblasts
Cell biology
Pregnancy Trimester, First
Endocrinology
medicine.anatomical_structure
nervous system
Pregnancy Trimester, Second
embryonic structures
Female
Cytotrophoblasts
Anatomy
Netrin Receptors
Cell Division
Subjects
Details
- ISSN :
- 15515044 and 00221554
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Journal of Histochemistry & Cytochemistry
- Accession number :
- edsair.doi.dedup.....fb11dced81955225592da78a289743a4