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Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis
- Source :
- Phytochemical Analysis. 14:150-154
- Publication Year :
- 2003
- Publisher :
- Wiley, 2003.
-
Abstract
- A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high-voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite-addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography–electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin-based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives. Copyright © 2003 John Wiley & Sons, Ltd.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Cyanidin
Acacia
Flowers
Plant Science
Mass spectrometry
Biochemistry
Proteaceae
Analytical Chemistry
Anthocyanins
Banksia
chemistry.chemical_compound
Pigment
Drug Discovery
Botany
Electrophoresis, Paper
Chromatography, High Pressure Liquid
Peonidin
Molecular Structure
biology
Chemistry
fungi
food and beverages
General Medicine
biology.organism_classification
Plant Leaves
Complementary and alternative medicine
Anthocyanin
visual_art
visual_art.visual_art_medium
Molecular Medicine
Delphinidin
Food Science
Subjects
Details
- ISSN :
- 10991565 and 09580344
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Phytochemical Analysis
- Accession number :
- edsair.doi.dedup.....fae030095739a262507125edf2379d6a