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Differential distribution of glutamate- and GABA-gated chloride channels in the housefly Musca domestica
- Source :
- Journal of Insect Physiology. 59:887-893
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- l-Glutamic acid (glutamate) mediates fast inhibitory neurotransmission by affecting glutamate-gated chloride channels (GluCls) in invertebrates. The molecular function and pharmacological properties of GluCls have been well studied, but not much is known about their physiological role and localization in the insect body. The distribution of GluCls in the housefly (Musca domestica L.) was thus compared with the distribution of γ-aminobutyric acid (GABA)-gated chloride channels (GABACls). Quantitative PCR and ligand-binding experiments indicate that the GluCl and GABACl transcripts and proteins are predominantly expressed in the adult head. Intense GluCl immunostaining was detected in the lamina, leg motor neurons, and legs of adult houseflies. The GABACl (Rdl) immunostaining was more widely distributed, and was found in the medulla, lobula, lobula plate, mushroom body, antennal lobe, and ellipsoid body. The present findings suggest that GluCls have physiological roles in different tissues than GABACls.
- Subjects :
- Male
biology
Physiology
Pupa
Glutamate receptor
Neurotransmission
Receptors, GABA-A
biology.organism_classification
Inhibitory postsynaptic potential
Cell biology
medicine.anatomical_structure
Biochemistry
Chloride Channels
Houseflies
Larva
Insect Science
Mushroom bodies
Chloride channel
medicine
Animals
Antennal lobe
Housefly
Immunostaining
Subjects
Details
- ISSN :
- 00221910
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Journal of Insect Physiology
- Accession number :
- edsair.doi.dedup.....fad2792e623e16ec47a643e6acc2cb0e
- Full Text :
- https://doi.org/10.1016/j.jinsphys.2013.06.005