Back to Search
Start Over
Apocynin Ameliorates Pressure Overload-Induced Cardiac Remodeling by Inhibiting Oxidative Stress and Apoptosis
- Source :
- Physiological Research. :741-752
- Publication Year :
- 2017
- Publisher :
- Institute of Physiology of the Czech Academy of Sciences, 2017.
-
Abstract
- Oxidative stress plays an important role in pressure overload-induced cardiac remodeling. The purpose of this study was to determine whether apocynin, a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, attenuates pressure overload-induced cardiac remodeling in rats. After abdominal aorta constriction, the surviving rats were randomly divided into four groups: sham group, abdominal aorta constriction group, apocynin group, captopril group. Left ventricular pathological changes were studied using Masson’s trichrome staining. Metalloproteinase-2 (MMP-2) levels in the left ventricle were analyzed by western blot and gelatin zymography. Oxidative stress and apoptotic index were also examined in cardiomyocytes using dihydroethidium and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), respectively. Our results showed that abdominal aorta constriction significantly caused excess collagen deposition and cardiac insult. Treatment with apocynin significantly inhibited deposition of collagen and reduced the level of MMP-2. Furthermore, apocynin also decreased the NADPH oxidase activity, reactive oxygen species production and cardiomyocyte apoptotic index. Interestingly, apocynin only inhibited NADPH oxidase activity without affecting its expression or the level of angiotension II in the left ventricle. In conclusion, apocynin reduced collagen deposition, oxidative stress, and inhibited apoptosis, ultimately ameliorating cardiac remodeling by mechanisms that are independent of the renin-angiotensin system.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Physiology
Apoptosis
Blood Pressure
030204 cardiovascular system & hematology
medicine.disease_cause
Antioxidants
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Heart Rate
Internal medicine
medicine
Animals
chemistry.chemical_classification
Pressure overload
Reactive oxygen species
TUNEL assay
Ventricular Remodeling
Acetophenones
General Medicine
Angiotensin II
Rats
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
Terminal deoxynucleotidyl transferase
Apocynin
cardiovascular system
Reactive Oxygen Species
Nicotinamide adenine dinucleotide phosphate
Oxidative stress
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 18029973 and 08628408
- Database :
- OpenAIRE
- Journal :
- Physiological Research
- Accession number :
- edsair.doi.dedup.....facf5fbdaf025187baafb0ab7ea16d29