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Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells
- Source :
- Materials scienceengineering. C, Materials for biological applications. 114
- Publication Year :
- 2020
-
Abstract
- P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed protamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP1880/LMWF8775 CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.
- Subjects :
- Gadolinium DTPA
Materials science
Endothelium
MRI contrast agent
Contrast Media
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
chemistry.chemical_compound
Polysaccharides
medicine
Cytotoxicity
biology
Fucoidan
Endothelial Cells
021001 nanoscience & nanotechnology
Protamine
Magnetic Resonance Imaging
In vitro
0104 chemical sciences
medicine.anatomical_structure
chemistry
Mechanics of Materials
Drug delivery
biology.protein
Cell-penetrating peptide
Biophysics
Nanoparticles
0210 nano-technology
Peptides
Subjects
Details
- ISSN :
- 18730191
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Materials scienceengineering. C, Materials for biological applications
- Accession number :
- edsair.doi.dedup.....facb7d35fdfe6e72c7eba72b4b5429d9