Back to Search
Start Over
Contribution of PDGFRα lineage cells in adult mouse hematopoiesis
- Source :
- Biochemical and biophysical research communications. 534
- Publication Year :
- 2020
-
Abstract
- Platelet-derived growth factor receptor alpha (PDGFRα) is a dominant marker of mesodermal mesenchymal cells in mice. Previous studies demonstrated that PDGFRα-positive (PDGFRα+) mesodermal cells develop not only into mesenchymal cells but also into a subset of total hematopoietic cells (HCs) in the limited period during mouse embryogenesis. However, the precise characteristics of the PDGFRα lineage positive (PDGFRα Lin+) HCs in adult mouse hematopoiesis are largely unknown. In this study, we systematically evaluated the characteristics of PDGFRα Lin+ HCs in the bone marrow and peripheral blood using PDGFRα-CRE; ROSAtdTomato mice. Flow cytometry analysis revealed that PDGFRα Lin+ HCs accounted for approximately 20% of total HCs in both the bone marrow and peripheral blood in adult mice. Compositions of myeloid and lymphoid subpopulations among CD45+ mononuclear cells were almost identical in both PDGFRα Lin+ and PDGFRα Lin− cells. Single-cell RNA-sequencing analysis also demonstrated that the transcriptomic signatures of the PDGFRα Lin+ HCs in the peripheral blood largely overlapped with those of the PDGFRα Lin− HCs, suggesting equivalent functions of the PDGFRα Lin+ and PDGFRα Lin− HCs. Although pathophysiological activities of the PDGFRα Lin + HCs were not evaluated, our data clearly demonstrate a significant role of the PDGFRα Lin + HCs in physiological hematopoiesis in adult mice.
- Subjects :
- 0301 basic medicine
Male
Mesoderm
Myeloid
Receptor, Platelet-Derived Growth Factor alpha
Biophysics
Biology
behavioral disciplines and activities
Biochemistry
Peripheral blood mononuclear cell
Flow cytometry
03 medical and health sciences
Mice
0302 clinical medicine
Growth factor receptor
mental disorders
medicine
Animals
Cell Lineage
RNA-Seq
Molecular Biology
medicine.diagnostic_test
fungi
Mesenchymal stem cell
Cell Biology
Hematopoietic Stem Cells
Hematopoiesis
Haematopoiesis
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
embryonic structures
Cancer research
Female
sense organs
Bone marrow
Single-Cell Analysis
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 534
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....fabcb9f5987bb8db8e279592a150e1cf