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Comparing the scoring mechanisms of p16INK4a immunohistochemistry based on independent nucleic stains and independent cytoplasmic stains in distinguishing between endocervical and endometrial adenocarcinomas in a tissue microarray study
- Source :
- Archives of Gynecology and Obstetrics. 281:293-300
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Endocervical adenocarcinomas (ECAs) and endometrial adenocarcinomas (EMAs) are malignancies that affect the uterus; however, their biological behaviors are quite different. This distinction has clinical significance because the appropriate therapy may depend on the site of tumor origin. The purpose of this study is to evaluate two different scoring mechanisms of p16INK4a immunohistochemical (IHC) stain in distinguishing between primary ECAs and EMAs. A tissue microarray (TMA) was constructed using formalin-fixed, paraffin-embedded tissue from hysterectomy specimens, including 14 ECAs and 21 EMAs. Tissue array sections were stained with a commercially available antibody, p16INK4a. The avidin–biotin complex method was used to visualize antigens. The staining intensity and extent of the IHC reactions were evaluated using a semi-quantitative scoring system. Two scoring methods were defined on the following bases: (1) independent cytoplasmic staining alone, irrespective of nucleic stain (Method C) and (2) independent nucleic staining alone, irrespective of cytoplasmic staining. (Method N). Of the two scoring mechanisms for p16INK4a expression, Method N showed a significant difference (P = 0.015), but Method C showed no significant (P = 0.432) frequency differences in distinguishing between ECAs and EMAs. However, Method N had a higher overall accuracy rate (71.4%) in accurately diagnosing ECAs from EMAs in the total number of p16INK4a IHC cases. According to the data of p16INK4a expression in this TMA study, Method N is favorable and efficient in distinguishing between ECAs and EMAs, while Method C is not.
- Subjects :
- Cytoplasm
Pathology
medicine.medical_specialty
Uterus
Uterine Cervical Neoplasms
Adenocarcinoma
Biology
Sensitivity and Specificity
Stain
Statistics, Nonparametric
Antigen
Predictive Value of Tests
medicine
Humans
Clinical significance
Cyclin-Dependent Kinase Inhibitor p16
Retrospective Studies
Cell Nucleus
Tissue microarray
Reproducibility of Results
Obstetrics and Gynecology
General Medicine
Immunohistochemistry
Molecular biology
Endometrial Neoplasms
Staining
medicine.anatomical_structure
Nucleic acid
Female
Subjects
Details
- ISSN :
- 14320711 and 09320067
- Volume :
- 281
- Database :
- OpenAIRE
- Journal :
- Archives of Gynecology and Obstetrics
- Accession number :
- edsair.doi.dedup.....fab88991acfbec1ffbc1505f054e5c81