Chromatographic fractionation of antibiotic 136 and characterization of its components

Antibiotic 136, a mixture of five similar basic antibiotics, has been partially resolved by chromatography on activated carbon. Component A appeared identical with streptothricin by paper chromatography, elemental analysis, infrared spectrum and animal toxicity. Fraction ABCD could not be distinguished by paper chromatography from pleocidin. Fraction CDE showed chemical properties similar to the mixture streptolin AB. Among the acid hydrolysis products of Antibiotic 136 chromatographic fractions, l -β-lysine, α- d -gulosamine, streptolidine, and N-guan-streptoldyl- d -gulosaminide have been identified. Component D difers from components A, B, and C by containing an imidazoline structural unit differing from streptolidine. The general properties of the mixture Antibiotic 136 indicate that its components are members of the streptothricin complex of antibiotics.