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Quantification of Helicobacter pylori in gastric mucosa by real-time polymerase chain reaction: comparison with traditional diagnostic methods
- Source :
- Diagnostic Microbiology and Infectious Disease. 74:248-252
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- The aim of this study was to determine the main diagnostic validity parameters of a quantitative real-time polymerase chain reaction (PCR) system for detecting Helicobacter pylori in gastric biopsies. Prospective study. The real-time PCR has an internal control for eliminating the false negatives. Our system has a good diagnostic capacity compared with the gold standard and was superior in antral mucosa: area under the curve was 0.91 for antrum (95% confidence interval [CI] 0.87 to 0.96) and 0.83 for corpus (95% CI 0.77 to 0.9). The optimum cut-off point was 3.56 microorganisms/cell for antrum (sensitivity 83.5% [95% CI 74.2 to 89.9]; specificity 91.3% [95% CI 82.3 to 96.0]; positive predictive value 92.2%; negative predictive value 81.8%). The positive likelihood ratios were 9.61 and 8.52 for antrum and corpus, respectively. With the cut-off point that maximises the Youden index, 8.7% false positives were obtained. Our methodology is useful for diagnosing infection due to H. pylori and the false positives detected probably correspond to patients who were actually infected but the infection was not detected by traditional techniques. The clinical importance of these cases should be studied in greater detail since they may involve colonisations unrelated to the patient's digestive pathology.
- Subjects :
- Male
Microbiology (medical)
medicine.medical_specialty
Biopsy
Youden's J statistic
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Gastroenterology
Helicobacter Infections
Predictive Value of Tests
Internal medicine
False positive paradox
medicine
Humans
Prospective Studies
Antrum
Helicobacter pylori
biology
business.industry
General Medicine
Gold standard (test)
biology.organism_classification
Bacterial Load
Confidence interval
Infectious Diseases
Real-time polymerase chain reaction
Gastric Mucosa
Predictive value of tests
Female
business
Subjects
Details
- ISSN :
- 07328893
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Diagnostic Microbiology and Infectious Disease
- Accession number :
- edsair.doi.dedup.....faa23a011919ff2c64e7da8d01e167fb