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TBMS1 exerts its cytotoxicity in NCI-H460 lung cancer cells through nucleolar stress-induced p53/MDM2-dependent mechanism, a quantitative proteomics study

Authors :
Yingying Lin
Ji Xia
Fuquan Jiang
Yang Xu
Jie Liu
Dan Su
Guobin Xie
Source :
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1864:204-210
Publication Year :
2016
Publisher :
Elsevier BV, 2016.

Abstract

Tubeimoside-1 (TBMS1) exerts its anticancer effects by inducing G2/M arrest and apoptosis of cancer cells. However, the precise molecular mechanism of its anti-tumor effects has not been fully elucidated, especially the signaling pathways involved in the early stage of TBMS1 stimulation. In this study, we employed stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics approach and identified 439 proteins that exhibit significant differential expressions in NCI-H460 lung cancer cells upon exposure to TBMS1. Gene ontology and network analysis using DAVID and STRING on-line tools revealed that several nucleolar stress (ribosomal biogenesis) response proteins were differentially regulated by TBMS1. Functional validation demonstrated that TBMS1-induced NCI-H460 cell cytotoxicity involved nucleolar stress-induced p53/murine double minute clone 2 (MDM2), mTOR, and NF-κB signaling pathways.

Details

ISSN :
15709639
Volume :
1864
Database :
OpenAIRE
Journal :
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
Accession number :
edsair.doi.dedup.....faa232c7f0c3cbecfaae7fd67ccb1924