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Enhanced Fluorine-19 MRI Sensitivity using a Cryogenic Radiofrequency Probe: Technical Developments and Ex Vivo Demonstration in a Mouse Model of Neuroinflammation
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group UK, 2017.
-
Abstract
- Neuroinflammation can be monitored using fluorine-19 (19F)-containing nanoparticles and 19F MRI. Previously we studied neuroinflammation in experimental autoimmune encephalomyelitis (EAE) using room temperature (RT) 19F radiofrequency (RF) coils and low spatial resolution 19F MRI to overcome constraints in signal-to-noise ratio (SNR). This yielded an approximate localization of inflammatory lesions. Here we used a new 19F transceive cryogenic quadrature RF probe ( 19 F-CRP) that provides the SNR necessary to acquire superior spatially-resolved 19F MRI. First we characterized the signal-transmission profile of the 19 F-CRP. The 19 F-CRP was then benchmarked against a RT 19F/1H RF coil. For SNR comparison we used reference compounds including 19F-nanoparticles and ex vivo brains from EAE mice administered with 19F-nanoparticles. The transmit/receive profile of the 19 F-CRP diminished with increasing distance from the surface. This was counterbalanced by a substantial SNR gain compared to the RT coil. Intraparenchymal inflammation in the ex vivo EAE brains was more sharply defined when using 150 μm isotropic resolution with the 19 F-CRP, and reflected the known distribution of EAE histopathology. At this spatial resolution, most 19F signals were undetectable using the RT coil. The 19 F-CRP is a valuable tool that will allow us to study neuroinflammation with greater detail in future in vivo studies.
- Subjects :
- Pathology
medicine.medical_specialty
Materials science
Encephalomyelitis, Autoimmune, Experimental
Encephalomyelitis
lcsh:Medicine
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
RF probe
Article
030218 nuclear medicine & medical imaging
Fluorine-19 Magnetic Resonance Imaging
03 medical and health sciences
Mice
0302 clinical medicine
Nuclear magnetic resonance
Magnetic resonance imaging
In vivo
medicine
Image Processing, Computer-Assisted
Animals
lcsh:Science
Neuroinflammation
Multidisciplinary
medicine.diagnostic_test
lcsh:R
Experimental autoimmune encephalomyelitis
Brain
medicine.disease
Image Enhancement
Experimental models of disease
Disease Models, Animal
Cardiovascular and Metabolic Diseases
Nanoparticles
lcsh:Q
Technology Platforms
030217 neurology & neurosurgery
Ex vivo
Radiofrequency coil
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....fa9bf00fe095627c3779922e1bb73bc7