Back to Search
Start Over
Motivated Behaviors and Levels of 3α,5α-THP in the Midbrain Are Attenuated by Knocking Down Expression of Pregnane Xenobiotic Receptor in the Midbrain Ventral Tegmental Area of Proestrous Rats
- Source :
- The Journal of Sexual Medicine. 10:1692-1706
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Progesterone (P4 ) and its product, 5α-pregnan-3α-ol-20-one (3α,5α-THP), act in the midbrain ventral tegmental area (VTA) to alter motivated behaviors, such as mating, and motor and anxiety behavior. Of interest is whether 3α,5α-THP formation requires the pregnane xenobiotic receptor (PXR), which is expressed in the midbrain of rats.The role of PXR in the midbrain for 3α,5α-THP formation, which precedes modulation of motivated behaviors, was investigated.Rats had estrous cycle phase determined and were assessed when they were in diestrus or proestrus. Diestrous and proestrous rats were infused with control or antisense oligodeoxyribonucleotides (AS-ODNs) targeted against PXR to the VTA.In pilot studies, PXR gene and protein expression in the midbrain were determined with quantitative reverse transcriptase polymerase chain reaction and Western blotting, respectively. Diestrous and proestrous rats infused with control or AS-ODNs to the VTA were tested for anxiety (open field and plus maze), social (social interaction), and sexual (paced mating) behavior. Expression of PXR in the midbrain was verified with Western blotting. Plasma estradiol, P4 , dihydroprogesterone (DHP), and 3α,5α-THP levels, and brain P4 , DHP, and 3α,5α-THP levels were measured. We predicted that proestrous rats infused with PXR AS-ODNs would have decreased anti-anxiety, social, and sexual behavior, lower midbrain expression of PXR, and lower midbrain levels of 3α,5α-THP compared with controls.Results supported the hypothesis that formation of 3α,5α-THP requires PXR and may be important for motivated behaviors. PXR AS-ODN, compared with control, infusions to the VTA reduced PXR expression and 3α,5α-THP levels in the midbrain and attenuated sexual receptivity of proestrous rats.Knockdown of PXR in the midbrain reduces 3α,5α-THP levels and sexual receptivity of proestrous rats. Thus, PXR in the midbrain may be required for the observed increase in 3α-5α-THP during proestrus, which has subsequent effects on motivated, reproductive behaviors.
- Subjects :
- Male
Receptors, Steroid
medicine.medical_specialty
Neuroactive steroid
Urology
Endocrinology, Diabetes and Metabolism
Receptivity
Pregnanolone
Anxiety
Biology
Article
Midbrain
Sexual Behavior, Animal
chemistry.chemical_compound
Endocrinology
Mesencephalon
Internal medicine
medicine
Animals
Interpersonal Relations
Rats, Long-Evans
Progesterone
Motivation
Pregnane X receptor
Estradiol
Reproduction
Ventral Tegmental Area
Allopregnanolone
Pregnane
Pregnane X Receptor
Rats
Ventral tegmental area
Psychiatry and Mental health
medicine.anatomical_structure
nervous system
Reproductive Medicine
chemistry
Gene Knockdown Techniques
Female
Proestrus
Subjects
Details
- ISSN :
- 17436095
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- The Journal of Sexual Medicine
- Accession number :
- edsair.doi.dedup.....fa99f770dea511269bd8f206027a3634
- Full Text :
- https://doi.org/10.1111/jsm.12173