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Electrospray ionization quadrupole time-of-flight mass spectrometry and quadrupole mass spectrometry for genotyping single nucleotide substitutions in intact polymerase chain reaction products in K-ras and p53
- Source :
- Rapid Communications in Mass Spectrometry. 16:2278-2285
- Publication Year :
- 2002
- Publisher :
- Wiley, 2002.
-
Abstract
- Single nucleotide polymorphisms (SNPs) and mutations were genotyped for both homozygous and heterozygous PCR products of p53, a tumor suppressor gene, and K-ras, an oncogene, using electrospray ionization (ESI) quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) and ESI-quadrupole MS analysis. Mass accuracy was adequate for both instruments to detect genetic changes in homozygous PCR products, including the most difficult to distinguish (adenine [A] --> thymine [T] transversion). However, for the detection of A --> T shifts (9.0 Da difference) in heterozygous PCR products, the increased resolution of ESI-Q-TOFMS proved essential. Although, greater mass differences in heterozygotes (e.g. cytosine [C] T or guanine [G] A) can be discriminated using ESI-quadrupole MS analysis.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Genotype
Resolution (mass spectrometry)
Guanine
Electrospray ionization
Molecular Sequence Data
Mass spectrometry
Polymerase Chain Reaction
Mass Spectrometry
Analytical Chemistry
chemistry.chemical_compound
Humans
Point Mutation
Transversion
Spectroscopy
Chromatography
Base Sequence
Chemistry
Organic Chemistry
Sequence Analysis, DNA
Genes, p53
Molecular biology
Thymine
Genes, ras
Cytosine
DNA
Subjects
Details
- ISSN :
- 10970231 and 09514198
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Rapid Communications in Mass Spectrometry
- Accession number :
- edsair.doi.dedup.....fa96f6c830359d3d496e96eb8a3649fc