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Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella
- Source :
- Journal of Antimicrobial Chemotherapy, 63(2), 274-281, Journal of Antimicrobial Chemotherapy 63 (2009) 2
- Publication Year :
- 2009
-
Abstract
- Objectives: The aim of this study was to identify and characterize plasmids carrying qnrS1, qnrB2 and qnrB19 genes identified in Salmonella strains from The Netherlands. The identification of plasmids may help to follow the dissemination of these resistance genes in different countries and environments. Methods: Plasmids from 33 qnr-positive Salmonella strains were transferred to Escherichia coli and analysed by restriction, Southern blot hybridization, PCR and sequencing of resistance determinants. They were also assigned to incompatibility groups by PCR-based replicon typing, including three additional PCR assays for the IncU, IncR and ColE groups. The collection included isolates from humans and one from chicken meat. Results: Five IncN plasmids carrying qnrS1, qnrB2 and qnrB19 genes were identified in Salmonella enterica Bredeney, Typhimurium PT507, Kentucky and Saintpaul. qnrS1 genes were also located on three further plasmid types, belonging to the ColE (in Salmonella Corvallis and Anatum), IncR (in Salmonella Montevideo) and IncHI2 (in Salmonella Stanley) groups. Conclusions: Multiple events of mobilization, transposition and replicon fusion generate the complexity observed in qnr-positive isolates that are emerging worldwide. Despite the fact that the occurrence of qnr genes in bacteria from animals is scarcely reported, these genes are associated with genetic elements and located on plasmids that are recurrent in animal isolates.
- Subjects :
- Salmonella typhimurium
Salmonella
chloramphenicol
kanamycin
antibiotic resistance
Drug Resistance
mediated quinolone resistance
Quinolones
medicine.disease_cause
Genetic analysis
Polymerase Chain Reaction
DNA transposition
Plasmid
Pharmacology (medical)
Replicon
Southern
protein qnrB19
Antibacterial agent
Netherlands
Genetics
Recombination, Genetic
spectrum-beta-lactamase
biology
Southern blotting
Blotting
sulfamethoxazole
article
Bacterial
Salmonella enterica
unclassified drug
Anti-Bacterial Agents
Meat Products
Blotting, Southern
Infectious Diseases
protein qnrB2
Conjugation, Genetic
enterobacter-cloacae
Salmonella Infections
determinant qnrs1
Sequence Analysis
Plasmids
Microbiology (medical)
protein qnrS1
DNA, Bacterial
streptomycin
Evolution
united-states
gene sequence
gentamicin
minimum inhibitory concentration
Microbiology
Evolution, Molecular
Genetic
ciprofloxacin
enterica
plasmid
Drug Resistance, Bacterial
complete nucleotide-sequence
medicine
Escherichia coli
Animals
Humans
trimethoprim
Typing
modifying enzyme
ampicillin
multidrug resistance protein
nalidixic acid
tetracycline
unclassified drug, antibiotic resistance
bacterial strain
gene identification
nonhuman
nucleotide sequence
polymerase chain reaction
replicon
United States, Animals
Chickens
Genes, Bacterial
Sequence Analysis, DNA
Pharmacology
klebsiella-pneumoniae
Conjugation
Molecular
DNA
biology.organism_classification
United States
Recombination
Genes
escherichia-coli
CVI - Divisie Bacteriologie en TSE's
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 63
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....fa94f14d070604c7135319a716065eeb
- Full Text :
- https://doi.org/10.1093/jac/dkn470